0000000000595774

AUTHOR

Else Müller-schweinitzer

showing 2 related works from this author

Freezing without surrounding cryomedium preserves the endothelium and its function in human internal mammary arteries

2005

Abstract Purpose Cryopreserved human blood vessels may become important tools in bypass surgery. Optimal cryopreservation of an arterial graft should, therefore, preserve both histological and physiological characteristics of smooth muscle and endothelium comparable to the unfrozen artery. Methods Rings from human internal mammary arteries (IMA) were investigated in vitro either unfrozen or after immersion into a cryomedium (RPMI 1640 containing 1.8 M Me2SO and 0.1 M sucrose) and cryostorage with and without surrounding medium. Results In unfrozen IMA, neither contractile responses to noradrenaline (NA) nor endothelium-dependent relaxant responses to acetylcholine (ACH) was modified after e…

SucrosePathologymedicine.medical_specialtyEndotheliumBiologyGeneral Biochemistry Genetics and Molecular BiologyCryopreservationAndrologyNorepinephrinechemistry.chemical_compoundCryoprotective AgentsFreezingmedicineHumansDimethyl SulfoxideEndotheliumMammary ArteriesPhorbol 1213-DibutyrateProtein Kinase CProtein kinase CCryopreservationDose-Response Relationship DrugDimethyl sulfoxideTemperatureMuscle SmoothGeneral MedicineAcetylcholineCulture MediaCold TemperatureEnzyme ActivationMicroscopy ElectronDose–response relationshipmedicine.anatomical_structurechemistryCarcinogensMicroscopy Electron ScanningMammary arteryEndothelium VascularTissue PreservationGeneral Agricultural and Biological SciencesAcetylcholinemedicine.drugArteryCryobiology
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Effects of the novel polymer gel LeGoo on human internal thoracic arteries.

2011

Purpose Established hemostatic devices can injure vessel wall integrity. LeGoo (Pluromed, Woburn, MA), a novel poloxamer gel with reverse thermosensitive properties, is a new device for temporary occlusion of blood vessels. The present study investigated the effects of LeGoo on vascular function and morphology. Description The distal end of the human internal thoracic artery was used to assess vascular function of LeGoo-applied segments in organ bath experiments and by scanning electron microscopy. Evaluation After LeGoo application, both maximal contractile responses to noradrenaline and endothelium-dependent relaxant responses to acetylcholine were significantly reduced. Scanning electron…

Pulmonary and Respiratory MedicineMaleEndotheliumInternal thoracic arteryPoloxamerMuscle Smooth VascularNorepinephrinemedicine.arteryMedicineHumansNew devicePolymer gelThoracic arteryMammary ArteriesAgedbusiness.industryOrgan bathAnatomyMiddle AgedAcetylcholinemedicine.anatomical_structureSurgeryFemaleEndothelium VascularCardiology and Cardiovascular MedicinebusinessTemporary occlusionAcetylcholinemedicine.drugThe Annals of thoracic surgery
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