North African hybrid sparrows (Passer domesticus, P. hispaniolensis) back from oblivion – ecological segregation and asymmetric mitochondrial introgression between parental species
A stabilized hybrid form of the house sparrow (Passer domesticus) and the Spanish sparrow (P. hispaniolensis) is known as Passer italiae from the Italian Peninsula and a few Mediterranean islands. The growing attention for the Italian hybrid sparrow and increasing knowledge on its biology and genetic constitution greatly contrast the complete lack of knowledge of the long-known phenotypical hybrid sparrow populations from North Africa. Our study provides new data on the breeding biology and variation of mitochondrial DNA in three Algerian populations of house sparrows, Spanish sparrows, and phenotypical hybrids. In two field seasons, the two species occupied different breeding habitats: Spa…
Post-Messinian evolutionary relationships across the Sicilian channel: Mitochondrial and nuclear markers link a new green toad from Sicily to African relatives
19 páginas, 6 figuras y 3 tables
Differences in gene flow in a twofold secondary contact zone of pond turtles in southern Italy (Testudines: Emydidae:Emys orbicularis galloitalica,E. o. hellenica,E. trinacris)
Using virtually range-wide sampling for three pond turtle taxa (Emys orbicularis galloitalica, E. o. hellenica, E. trinacris), we analyse gene flow across their southern Italian contact zone. Based on population genetic analyses of 15 highly polymorphic microsatellite loci and a mitochondrial marker, we show that the general genetic pattern matches well with the current taxon delimitation. Yet, single individuals with conflicting genetic identity suggest translocation of turtles by humans. In addition, we identify in south-western France and the vicinity of Rome populations being heavily impacted by introduced turtles. Cline analyses reveal that the major genetic break between E. o. galloit…
Genetic admixture despite ecological segregation in a North African sparrow hybrid zone (Aves, Passeriformes, Passer domesticus × Passer hispaniolensis)
Under different environmental conditions, hybridization between the same species might result in different patterns of genetic admixture. Particularly, species pairs with large distribution ranges and long evolutionary history may have experienced several independent hybridization events over time in different zones of overlap. In birds, the diverse hybrid populations of the house sparrow (Passer domesticus) and the Spanish sparrow (Passer hispaniolensis) provide a striking example. Throughout their range of sympatry, these two species do not regularly interbreed; however, a stabilized hybrid form (Passer italiae) exists on the Italian Peninsula and on several Mediterranean islands. The spa…
Preliminary genetic characterisation of Southern Smooth Snake Coronella girondica (Serpentes, Colubridae) populations in Italy, with some considerations on their alpine distribution
The Southern smooth snake, Coronella girondica, is a small-sized colubrid found in Northwest Africa and Southwest Europe. Mitochondrial DNA-based studies showed that the species can be split into five clades: two from Northwest Africa (one Moroccan and one Tunisian-Algerian) and three from Europe (one in the south-west of the Iberian Peninsula, one in the south-east of Spain and one in the rest of the European range). With regards to Italy, to date, only two samples have been analysed both from the Province of Pisa, Tuscany, pointing at that fact that genetic characterisation of Italian populations is still lacking. Accordingly, we have increased the sampling coverage with 19 new samples fr…
Is the painted frog Discoglossus pictus a declining species in Italy? On the reliability of a distributional atlas approach
The conservation status of amphibians is often assessed using public databases because of a lack of up-to-date field data. However, it is crucial that this kind of data is used carefully, evaluating the reliability and the consistency of the information. In Italy, the conservation status of the painted frog, Discoglossus pictus, was recently assessed using public databases, and the outcome highlighted a worrying situation for the species. We analyzed the reliability of these conclusions and reassessed the status of the painted frog, taking into account its insular distribution and new data of species occurrence. Our results contrast with the previous analysis and show how an incautious use …
The spreading of the invasive italian wall lizard on Vulcano, the last island inhabited by the critically endangered aeolian wall lizard
[EN] The Aeolian Wall Lizard (Podarcis raffoneae) is an endemic species of the Aeolian Archipelago of Italy (Mediterranean Sea). Its distribution is limited to three islets and two relict populations on a relatively large island: Vulcano (a population on the summit of Gran Cratere volcano and another on Capo Grosso promontory). The critically endangered Aeolian Wall Lizard is threatened by the introduction of the Italian Wall Lizard (Podarcis siculus), which successfully competes and hybridizes with the endemic lizard. The invasive lizard is widespread on Vulcano, although the literature does not provide the exact distribution. Our first aim was updating the distribution of the Italian Wall…
Biometrical Analyses of a Sicilian Green Toad, Bufo siculus (Stöck et al. 2008), Population Living in Sicily (Italy)
Se realizo un estudio morfometrico de la estructura del sapo verde siciliano de la Reserva Natural "Monte Pellegrino" (norte-oeste de Sicilia). Un total de 666 individuos (354 machos y 312 hembras) fueron capturados desde el ano 2003 durante la epoca de reproduccion. Se encontraron diferencias estadisticamente significativas entre los dos sexos, donde el peso corporal fue el mejor parametro (hasta 92% de clasificacion correcta), seguido por la longitud corporal (hasta 90% de clasificacion correcta). El uso simultaneo de todos los caracteres examinados solo aumento un 1% la probabilidad de una correcta discriminacion del sexo. En comparacion con la dimension de otras poblaciones de sapos ver…
The Sicilian rock partridge: latest data on genetic integrity from four different relict areas
Sicily (Italy) hosts a “relict”, endemic population of the birds Alectoris graeca whitakeri commonly known as Sicilian Rock Partridge. In the last decades, due to the risk of restocking with other European and Asiatic species for hunting purpose, a study was carried out to investigate the potential risk of hybridisation. The mtDNA control-region and nuclear microsatellites were genotyped. Due to the importance of the species, samples were mainly characterized by feather and stool samples, and rarely by carcasses found in the environment, from year 2011 to 2012. A panel of 7 microsatellite loci was validated. Three multiplexes that allowed the simultaneous amplification of 3 microsatellites,…
New data about Chiropterofauna of the “Monte Pellegrino” Nature Reserve (Palermo, Italy)
This work increase the knowledge related to bat fauna present in the “Monte Pellegrino” Nature Reserve in the Palermo municipality. The acoustic sampling took place in the summer/autumn season using the D500X Bat detector in 22 samples. The identified signals were attributed to 8 species and one genus. The Taxa with the most passes in the reserve are: Tadarita teniotis followed by Pipistrellus kuhlii, Hypsugo savii (100% of Costancy), Pipistrellus pygmaeus, Pipistrellus pipistrellus, Plecotus sp., Miniopterus schreibersii and last with a single recording Rhinolophus ferrumequinum and Rhinolophus hipposideros. The dendrogram of similarity shows 3 distinct bats cenosis. Two samples present in…
A case of cannibalism in the false smooth snake Macroprotodon cucullatus on the island of Lampedusa
A case of cannibalism in the false smooth snake Macroprotodon cucullatus on the island of Lampedusa
mtDNA diversity in rabbit population from Sicily (Italy)
The European rabbit Oryctolagus cuniculus (O.c) lives all over the world and it represents an important resource for many predators. It has been classified as a Near-Threatened species in the Red List of Vertebrates of Italy. It is present in mediterranean basin as two known subspecies: O.c. cuniculus and O.c. algirus. The mediterranean geographic distribution of the two subspecies is still not well known. In particular, in Sicily, lacking of deep studies, is based on the body size and morphological characteristics; there wasn’t a complete description of the actual existing subspecies and previous studies only reported the morphological characteristics of the sicilian rabbit population. In …
Census of the Sicilian rock partridge Alectoris graeca whitakeri population in ZPS ITA010029 Monte Cofano, Capo San Vito and Monte Sparagio
During the project "LIFE09 NAT/IT/000099-SICALECONS-Urgent actions for the conservation of Alectoris graeca whitakeri", this species was censused in the ZPS ITA010029 Monte Cofano, Capo San Vito and Monte Sparagio (Trapani province, Sicily) with playback technique between March and April 2011. Superimposing a UTM grid of 1 km mesh on the study area produced cells of 100 hectares. Using a GPS satellite receiver we reached, where possible, the centre of each cell from where the cospecific call was broadcasted. A total of 163 listening stations were implemented. Using software GIS ARCMAP 10.0 (ESRI) presence data were interpolated with vegetational and phytosociological maps (CORINE) to establ…
Conservation of the endangered Mediterranean tortoise Testudo hermanni hermanni: The contribution of population genetics and historical demography
International audience; Estimating the genetic variation and demographic trends of species in decline is of major concern in conservation genetics. This study contributes to understanding how historical and anthropogenic factors shape the distribution of current genetic diversity in one of the most endangered reptiles in Western Europe, the Hermann's tortoise Testudo hermanni hermanni. We used 17 microsatellite loci, chosen from a pyrosequencing library specifically developed for the subspecies to genotype eight populations distributed over about 30 sample localities covering almost the entire geographic distribution of the sub-species. The population genetic results reflect a very strong g…
Mitochondrial heteroplasmy in an avian hybrid form (Passer italiae: Aves, Passeriformes)
Mitochondrial heteroplasmy is the result from biparental transmission of mitochondrial DNA (mtDNA) to the offspring. In such rare cases, maternal and paternal mtDNA is present in the same individual. Though recent studies suggested that mtDNA heteroplasmy might be more common than previously anticipated, that phenomenon is still poorly documented and was mostly detected in case studies on hybrid populations. The Italian sparrow, Passer italiae is a homoploid hybrid form that occurs all across the Italian Peninsula mostly under strict absence of either of its parent species, the house sparrow (P. domesticus) and the Spanish sparrow (P. hispaniolensis). In this study, we document a new case o…