0000000000667002
AUTHOR
O. Hänninen
Toxicity and mutagenicity of waste waters from Baikalsk Pulp and Paper Mill: Evaluation of pollutant contamination in Lake Baikal
Lake Baikal has no agricultural and only little municipal pollution. Instead a potential source of pollution is the Baikalsk Pulp and Paper Mill (BPPM). All waste waters of the mill are mechanically, biologically and chemically purified and there are sedimentation and aeration ponds at the final stage. In this study the efficiency of the waste water purification was detected by testing toxicity and mutagenicity. Waters and sediments were collected along the cellulose bleaching process and waste water treatment. Free and bound polychlorinated phenols, guaiacols, catechols, anisoles and veratroles were analysed. Cytotoxicity of diethylether-extracted waste waters was screened by measuring tot…
Glutathione metabolism in skeletal muscle derived cells of the L6 line
Skeletal muscle derived L6 myoblasts possess a considerably high resting total glutathione (TGSH) pool. Exposure to L-buthionine-[S,R]-sulphoximine resulted in a 90% depletion of the intracellular TGSH pool. All the key enzymes of glutathione metabolism, especially glutathione S-transferase, were observed to be considerably active in the undifferentiated cells. Se-dependent glutathione peroxidase activity appeared to account for most of the total GSH peroxidase activity of the cells. A significant contribution of gamma-glutamyl transpeptidase-independent (5 mM acivicin insensitive) mechanism to the extracellular GSH uptake capacity of the muscle cells was evident. Efflux of oxidized glutath…