0000000000684316

AUTHOR

Zen-ichi Yamazaki

Die Wirkung einiger Cytostatica auf die DNA Synthese in einem oncogenen RNA Virus

Sowohl durch matrizeninaktivierende Cytostatica als auch durch Cytostatica, die am Enzymprotein angreifen, konnen in vitro die RNA-abhangige DNA-Polymerase und die DNA-abhangige DNA-Polymerase aus RML-Viren gehemmt werden. Von den getesteten Cytostatica, die die Matrize inaktivieren, wirken Daunoblastin (=Daunomycin) und Adriamycin selektiv hemmend auf die RNA-abhangige DNA-Polymerase, wahrend Olivomycin, Chromomycin und Actinomycin D bereits in niedrigen Konzentrationen die DNA-abhangige DNA-Polymerase aus RML-Viren selektiv inhibieren. Durch 2 Cytostatica konnen die enzymatischen Reaktionen der DNA-Polymerasen aus RML-Viren, durch Angriff am Enzymprotein beeinflust werden: Demethyl-Rifamp…

research product

Bleomycin, a selective inhibitor of DNA-dependent DNA polymerase from oncogenic RNA viruses.

Abstract Bleomycin, an antibiotic, inhibits the DNA-dependent DNA polymerase from Rauscher murine leukemia virus. Higher concentrations of BLM ∗ are required to inhibit it's RNA-dependent DNA polymerase. These inhibition effects of the non-competitive type are not altered by preincubation of the DNA with BLM. Under comparable conditions neither the DNA-dependent DNA polymerase activity from E. coli and mouse liver nor the DNA-dependent RNA polymerase activity from mouse lymphoma cells are affected by BLM.

research product

RNA dependent DNA polymerase in cells of xeroderma pigmentosum

Abstract Cells from X.P. ∗ skin contain an RNA dependent DNA polymerase, while in cells from normal skin this enzyme is lacking. This finding stimulates the thought that carcinogenesis in X.P. cells is due to an infection with an oncogenic RNA virus.

research product