0000000000718825

AUTHOR

Iva Neshkova

showing 3 related works from this author

Non-viral VEGF(165) gene therapy--magnetofection of acoustically active magnetic lipospheres ('magnetobubbles') increases tissue survival in an overs…

2008

Abstract Adenoviral transduction of the VEGF gene in an oversized skin flap increases flap survival and perfusion. In this study, we investigated the potential of magnetofection of magnetic lipospheres containing VEGF165-cDNA on survival and perfusion of ischemic skin flaps and evaluated the method with respect to the significance of applied magnetic field and ultrasound. We prepared perfluoropropane-filled magnetic lipospheres (‘magnetobubbles’) from Tween60-coated magnetic nanoparticles, Metafectene, soybean-oil and cDNA and studied the effect in an oversized random-pattern-flap model in the rats (n= 46). VEGF-cDNA-magnetobubbles were administered under a magnetic field with simultaneousl…

MalePathologymedicine.medical_specialtyNecrosismagneticAngiogenesisGenetic enhancementDermatologic Surgical ProceduresEnzyme-Linked Immunosorbent AssayTransfectionSurgical FlapsRats Sprague-DawleyMagneticsangiogenesismagnetobubblesmedicineAnimalsUltrasonicsSkinbusiness.industryVascular Endothelial Growth FactorsMusclesUltrasoundGraft SurvivalCell BiologyTransfectionGenetic TherapySkin TransplantationArticlesequipment and suppliesLipidsVEGFgene therapyMicrospheresRatsMicrovesselsModels AnimalMagnetofectionMolecular MedicineMagnetic nanoparticleslipospheresmedicine.symptombusinessPerfusionhuman activitiesmagnetofectionJournal of cellular and molecular medicine
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Magnetofection of acoustically active „magnetic microspheres“ (Magnetobubbles) — Induction of angiogenesis via non-viral transfection of VEGF

2009

Adenoviral transduction of the VEGF gene in an oversized skin flap increases flap survival and perfusion. In this study we investigated the potential of magnetofection of magnetic lipospheres containing VEGF165-cDNA on survival and perfusion of ischemic skin flaps and evaluated the method with respect to the significance of applied magnetic field and ultrasound.

integumentary systembiologybusiness.industryChemistryAngiogenesisVEGF receptorsUltrasoundSkin flapequipment and suppliesNon viral transfectioneye diseasesMicrospherebiology.proteinMagnetofectionCancer researchbusinesshuman activitiesPerfusion
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Searching for the right timing of surgical delay: angiogenesis, vascular endothelial growth factor and perfusion changes in a skin-flap model.

2009

Summary Background The angiogenic potential of vascular endothelial growth factor (VEGF) and its oxygen pressure-dependent regulation suggest a strong connection between this growth factor and the ‘delay phenomenon'. In this study we focused on the chronological changes in VEGF concentration and flap perfusion in order to optimise the duration of surgical delay. Methods The VEGF concentration in skin and underlying muscle was measured in oversized, random-pattern flaps on 38 male Sprague-Dawley rats after 3, 5 or 7 days of surgical delay. Additionally, flaps were raised 5 or 7 days past preconditioning. The effect on flap perfusion was measured using indocyanine green fluoroscopy and the si…

CD31Graft RejectionMalemedicine.medical_specialtyTime FactorsAngiogenesismedicine.medical_treatmentNeovascularization PhysiologicVasodilationEnzyme-Linked Immunosorbent AssayStatistics NonparametricSurgical FlapsNeovascularizationRats Sprague-Dawleychemistry.chemical_compoundRandom AllocationLaser-Doppler FlowmetryMedicineAnimalsProbabilitySkinbusiness.industryVascular Endothelial Growth FactorsGrowth factorMicrocirculationGraft SurvivalSkin TransplantationSurgeryRatsVascular endothelial growth factorDisease Models AnimalchemistryMultivariate AnalysisSurgerymedicine.symptombusinessIndocyanine greenPerfusionBiomarkersJournal of plastic, reconstructiveaesthetic surgery : JPRAS
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