0000000000741087

AUTHOR

Sáez G T

showing 2 related works from this author

A Simple Microassay for the Determination of Hydrazine in Biological Samples. Effect of Hydrazine and Isoniazid on Liver and Brain Glutathione

1987

A simple microassay for the determination of hydrazine in laboratory samples is presented. The colored product of the reaction of p-dimethylaminobenzaldehyde with hydrazine was tested at 470 nm using double-beam mode in different samples. Internal standards and data on blood serum, liver, and brain of rats treated with hydrazine or isoniazid are presented. The tissue glutathione content of these rats was determined, and the possible implication of glutathione in the brain toxicity of hydrazine is discussed.

MaleHealth Toxicology and MutagenesisMetaboliteToxicologyAnalytical Chemistrychemistry.chemical_compoundBlood serumSpectrophotometryIsoniazidmedicineAnimalsEnvironmental ChemistryHydrazine (antidepressant)Brain ChemistryChemical Health and SafetyChromatographymedicine.diagnostic_testIsoniazidMetabolismGlutathioneGlutathioneRatsHydrazinesLiverchemistryBiochemistryToxicitymedicine.drugJournal of Analytical Toxicology
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Age-Related Changes of Liver Antioxidant Enzymes and 8-Hydroxy-2-Deoxyguanosine During Fetal–Neonate Transition and Early Rat Development

2000

We have studied the pro-antioxidant status of the rat liver on the last day of gestation and at 1, 15, and 30 days of extrauterine life. Representative variables, such as activities of superoxide dismutase (SOD), catalase, and glutathione peroxidase and concentrations of reduced glutathione and 8-hydroxy-2'-deoxyguanosine, were determined in liver to assess the degree of birth-associated oxidative stress during the fetal-neonatal transition and early development of the rat. Percentages by which liver Cu/ZnSOD activity increased over the basal value of the fetal liver were 54%, 95%, and 127% at neonatal days 1, 15, and 30, respectively. There was a lack of induction in the development profil…

Agingmedicine.medical_specialtyAntioxidantmedicine.medical_treatmentClinical Biochemistrymedicine.disease_causeBiochemistryAntioxidantsSuperoxide dismutasechemistry.chemical_compoundFetusPregnancyInternal medicineGeneticsmedicineAnimalsDeoxyguanosineRats WistarMolecular Biologychemistry.chemical_classificationGlutathione PeroxidaseFetusbiologySuperoxide DismutaseGlutathione peroxidaseDeoxyguanosineCell BiologyGlutathioneCatalaseGlutathioneRatsOxidative StressEndocrinologyAnimals NewbornLiverchemistry8-Hydroxy-2'-DeoxyguanosineCatalasebiology.proteinFemaleOxidative stressDNA DamageIUBMB Life (International Union of Biochemistry and Molecular Biology: Life)
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