0000000000765947

AUTHOR

Vera Jakobi

showing 4 related works from this author

Differential expression of Cryptosporidium parvum genes encoding sporozoite surface antigens in infected HCT-8 host cells.

2006

Intracellular replication of Cryptosporidium parvum (Apicomplexa) involves the generation of several asexual and sexual forms of the parasite. During the stage conversions, complex mechanisms lead to differential structural and functional properties of the parasite. These require a well tuned gene transcription machinery. For the first time the gene expression of four surface proteins of C. parvum sporozoites, CP15, CP17, P23, and GP900 were analysed in parallel by reverse transcription polymerase chain reaction. In addition, CP17 and P23 antigens were detected in infected host cells by immunofluorescence using antisera raised against recombinant forms of the proteins. The results show that…

CytoplasmTime FactorsTranscription GeneticImmunologyGenes ProtozoanProtozoan ProteinsFluorescent Antibody TechniqueAntigens ProtozoanBiologyImmunofluorescenceMicrobiologyApicomplexaAntigenCell Line Tumorparasitic diseasesGene expressionmedicineAnimalsHumansRNA MessengerGeneCryptosporidium parvumMembrane Glycoproteinsmedicine.diagnostic_testReverse Transcriptase Polymerase Chain Reactionbiology.organism_classificationMolecular biologyAdaptation PhysiologicalReverse transcription polymerase chain reactionInfectious DiseasesReal-time polymerase chain reactionCryptosporidium parvumGene Expression RegulationAntigens SurfaceRNA ProtozoanMicrobes and infection
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Binding and activation of human and mouse complement by Cryptosporidium parvum (Apicomplexa) and susceptibility of C1q- and MBL-deficient mice to inf…

2008

Cryptosporidium parvum is a protozoan parasite (Apicomplexa) that causes gastrointestinal disease in animals and humans. Whereas immunocompetent hosts can limit the infection within 1 or 2 weeks, immunocompromised individuals develop a chronic, life-threatening disease. The importance of the adaptive cellular immune response, with CD4+ T-lymphocytes being the major players, has been clearly demonstrated. Several non-adaptive immune mechanisms have been suggested to contribute to the host defence, such as interferon-gamma (IFN-gamma) from NK cells, certain chemokines, beta-defensins and pro-inflammatory cytokines, but the influence of the complement systems has been less well studied. We ana…

ChemokineImmunologyProtozoan ProteinsCryptosporidiosisComplement factor ISodium ChlorideMannose-Binding LectinMicrobiologyMiceImmune systemmedicineAnimalsHumansRNA MessengerMolecular BiologyComplement ActivationImmunodeficiencyMannan-binding lectinCryptosporidium parvumbiologyReverse Transcriptase Polymerase Chain ReactionComplement C1qOocystsTemperaturemedicine.diseasebiology.organism_classificationVirologyComplement systemMice Inbred C57BLCryptosporidium parvumGene Expression RegulationLectin pathwayComplement C3bbiology.proteinCattleDisease SusceptibilityMolecular immunology
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Host immune response to Cryptosporidium parvum infection

2010

Species of the genus Cryptosporidium are protozoan parasites (Apicomplexa) that cause gastroenteritis in animals and humans. Of these Cryptosporidium parvum and Cryptosporidium hominis are the major causative agents of human cryptosporidiosis. Whereas infection is self-limiting in the immunocompetent hosts, immunocompromised individuals develop a chronic, life-threatening disease. As specific therapeutic or preventive interventions are not yet available, better understanding of the immune response to the parasite is required. This minireview briefly summarizes the factors involved in the innate and acquired immune response in this pathogen-host interaction with an emphasis on more recent da…

T-Lymphocytesanimal diseasesAIDS-Related Opportunistic InfectionsImmunologyAntibodies ProtozoanCryptosporidiosisAdaptive ImmunityBiologyNitric OxideImmunocompromised HostMiceImmune systemIntestinal mucosaImmunityparasitic diseasesAnimalsHumansIntestinal MucosaCryptosporidium parvumB-LymphocytesPhagocytesAIDS-Related Opportunistic InfectionsComplement System ProteinsDendritic CellsGeneral MedicineAcquired immune systembiology.organism_classificationVirologyImmunity InnateKiller Cells NaturalDisease Models AnimalInfectious DiseasesCryptosporidium parvumImmunologyCytokinesParasitologyImmunocompetenceImmunocompetenceCryptosporidium hominisExperimental Parasitology
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Dynamics of gut mucosal and systemic Th1/Th2 cytokine responses in interferon-gamma and interleukin-12p40 knock out mice during primary and challenge…

2009

Cryptosporidium parvum is an intracellular parasite causing enteritis which can become life-threatening in the immunocompromised host. CD4+ T cells and interferon (IFN)-gamma play dominant roles in host immune response to infection. However, effector mechanisms that are responsible for recovery from infection are poorly understood. In the present study we analyzed mice deficient in IFN-gamma or interleukin (IL)-12 in parallel to C57BL/6 wild type mice as models for murine cryptosporidiosis. Our results identified IFN-gamma as the key cytokine in the innate as well as adaptive immunity during primary and also challenge C. parvum infection. Furthermore, both Th1 and Th2 cytokines appear to co…

medicine.medical_treatmentImmunologyCryptosporidiosisBiologyInterferon-gammaMiceImmune systemTh2 CellsImmunityIleummedicineImmunology and AllergyAnimalsInterferon gammaCryptosporidium parvumMice KnockoutInterleukin-12 Subunit p40Interleukin-18InterleukinHematologyTh1 CellsAcquired immune systembiology.organism_classificationMice Inbred C57BLCytokineCryptosporidium parvumGene Expression RegulationGastric MucosaOrgan SpecificityImmunologyInterleukin 12medicine.drugImmunobiology
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