0000000000770837

AUTHOR

Ursula Egner

showing 2 related works from this author

Chromogranin A in the Mammalian Merkel Cell: Cellular and Subcellular Distribution

1989

Chromogranin-A (CGA), which accounts for more than half the soluble matrix protein in secretory granules of various neuroendocrine cells, has a wide spectrum of potential biological roles and is considered an important marker of the diffuse neuroendocrine system (DNES). Light and electron microscopic immunohistochemistry of mammalian skin revealed that Merkel cells are exclusively CGA-immunoreactive (ir) and that the immunoreaction is localized in the secretory granules. This finding supports the classification of the Merkel cell as a member of the DNES. The CGA immunoreactivity was restricted to Merkel cells of pigs and humans. In human embryonic skin, CGA was expressed in Merkel cells as …

endocrine systemPathologymedicine.medical_specialtySwineVasoactive intestinal peptideNerve Tissue ProteinsDermatologyHorseradish peroxidaseBiochemistryImmunoenzyme TechniquesmedicineChromograninsAnimalsHumansMolecular BiologyViral matrix proteinintegumentary systembiologyAge FactorsChromogranin ACell BiologyEmbryonic stem cellCell biologyCell CompartmentationMicroscopy Electronmedicine.anatomical_structureEpidermal Cellsbiology.proteinUltrastructureImmunohistochemistryChromogranin AEpidermisMerkel cellJournal of Investigative Dermatology
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Electron microscopic immunogold cytochemistry reveals chromogranin A confined to secretory granules of porcine Merkel cells

1990

By ultrastructural immunohistochemistry using the immunogold technique, immunoreactive (ir) Chromogranin A (CGA) was found to be confined to the secretory vesicles of porcine Merkel cells. CGA was present predominantly in the periphery of the electron-dense core and on the clear halo. These findings indicate that CGA is a regular constituent of Merkel cell secretory granules but probably not exclusively responsible for their electron opacity.

endocrine systemPathologymedicine.medical_specialtySwineCytoplasmic GranulesCalcium-binding proteinChromograninsmedicineAnimalsSkinintegumentary systembiologyGeneral NeuroscienceChromogranin AImmunogold labellingImmunohistochemistryNeurosecretory SystemsMolecular biologySecretory VesicleMicroscopy Electronmedicine.anatomical_structurebiology.proteinCytochemistryUltrastructureChromogranin AImmunohistochemistryGoldMerkel cellNeuroscience Letters
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