0000000000771064

AUTHOR

Bjarne Holmbom

showing 3 related works from this author

Wood-derived estrogens: studies in vitro with breast cancer cell lines and in vivo in trout.

1996

The wood-derived compound, beta-sitosterol (purity > 90%), was shown to be estrogenic in fish. It induced the expression of the vitellogenin gene in the liver of juvenile and methyltestosterone-treated rainbow trout. Structural similarities to beta-sitosterol notwithstanding, cholesterol, citrostadienol, beta-sitostanol, and 5-androstene-3 beta,17 beta-diol, an estrogenic member of the androstenic steroid group, were inactive. An abietic acid mixture (37% abietic acid, 6% dehydroabietic acid, and a remainder of unknown compounds) showed slight hormonal activity in feed, but it was completely inactive when given intraperitoneally in implants. The estrogenic component of the abietic acid prep…

medicine.medical_specialtymedicine.drug_classIsorhapontigeninPinosylvinEstrogen receptorGene ExpressionBreast NeoplasmsToxicologyVitellogeninchemistry.chemical_compoundVitellogeninsInternal medicinemedicineTumor Cells CulturedBioassayAnimalsHumansAbietic acidPharmacologyBetulinbiologyPhytosterolsEstrogensBlotting NorthernWoodEndocrinologychemistryEstrogenOncorhynchus mykissbiology.proteinRNACell DivisionWater Pollutants ChemicalToxicology and applied pharmacology
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PCA and PLS methods applied to ecotoxicological data: Ecobalance project

1996

Within a case study ‘Ecobalance’, the fate and effects of various chlorinated and non-chlorinated organic compounds and some heavy metals discharged from pulp and paper mills into water, sediment and aquatic animals were studied in a recipient area of southern Lake Saimaa, SE Finland. The main aim of the project was to find an empirical link between chemical emission parameters and ecotoxicological effects expressed in the ecosystem. As part of the \‘Ecobalance’ project, principal component analysis (PCA) and partial least squares (PLS) methods were used to interpret the data of the lake area. The PLS method was used to estimate the lake area affected by mill effluents and to calculate the …

Pulp millHydrologyPollutionApplied Mathematicsmedia_common.quotation_subjectAnalytical ChemistryWastewaterEnvironmental chemistryPartial least squares regressionPrincipal component analysisEnvironmental scienceEcotoxicologyEcosystemEffluentmedia_commonJournal of Chemometrics
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Expression of the Vitellogenin Gene in the Liver of Juvenile Whitefish (Coregonus lavaretus L. s.l.) Exposed to Effluents from Pulp and Paper Mills

1999

Juvenile whitefish (Coregonus lavaretus L. s.l.) were exposed by caging in the field to diluted effluents from three operating pulp, paper, and paperboard mills in Southern Lake Saimaa, Finland. The expression of the vitellogenin gene, used as a biomarker of estrogenic contamination of effluents, was measured using a Northern blotting method. Increased mRNA levels, the most specific and reliable evidence for estrogen receptor-mediated actions in vivo, were found in fish caged in the vicinity of one of three mills studied. This mill was found to discharge wood-derived compounds, such as sterols and resin acids, into Lake Saimaa in amounts considerably exceeding those from the other two mills…

PaperHealth Toxicology and MutagenesisIndustrial WasteZoologyBiologycomplex mixturesVitellogeninsVitellogeninfoodCoregonus lavaretusGene expressionAnimalsJuvenileRNA MessengerNorthern blotEffluentFinlandSalmonidaePollutantfood.dishEcologydigestive oral and skin physiologytechnology industry and agriculturePublic Health Environmental and Occupational HealthGeneral MedicineBlotting Northernbiology.organism_classificationWoodPollutionGene Expression RegulationLiverOncorhynchus mykissbiology.proteinSalmonidaeWater Pollutants ChemicalEcotoxicology and Environmental Safety
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