6533b856fe1ef96bd12b314b
RESEARCH PRODUCT
Expression of the Vitellogenin Gene in the Liver of Juvenile Whitefish (Coregonus lavaretus L. s.l.) Exposed to Effluents from Pulp and Paper Mills
Aimo OikariRisto SanttiBjarne HolmbomPirkko MellanenMarkus Soimasuosubject
PaperHealth Toxicology and MutagenesisIndustrial WasteZoologyBiologycomplex mixturesVitellogeninsVitellogeninfoodCoregonus lavaretusGene expressionAnimalsJuvenileRNA MessengerNorthern blotEffluentFinlandSalmonidaePollutantfood.dishEcologydigestive oral and skin physiologytechnology industry and agriculturePublic Health Environmental and Occupational HealthGeneral MedicineBlotting Northernbiology.organism_classificationWoodPollutionGene Expression RegulationLiverOncorhynchus mykissbiology.proteinSalmonidaeWater Pollutants Chemicaldescription
Juvenile whitefish (Coregonus lavaretus L. s.l.) were exposed by caging in the field to diluted effluents from three operating pulp, paper, and paperboard mills in Southern Lake Saimaa, Finland. The expression of the vitellogenin gene, used as a biomarker of estrogenic contamination of effluents, was measured using a Northern blotting method. Increased mRNA levels, the most specific and reliable evidence for estrogen receptor-mediated actions in vivo, were found in fish caged in the vicinity of one of three mills studied. This mill was found to discharge wood-derived compounds, such as sterols and resin acids, into Lake Saimaa in amounts considerably exceeding those from the other two mills. The increased vitellogenin gene expression suggests that the effluent is a source of estrogenic contaminants.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-06-01 | Ecotoxicology and Environmental Safety |