0000000000773515

AUTHOR

Tom Resler

showing 2 related works from this author

Kinetics of proton release and uptake by channelrhodopsin-2

2012

Electrophysiological experiments showed that the light-activated cation channel channelrhodopsin-2 (ChR2) pumps protons in the absence of a membrane potential. We determined here the kinetics of transient pH change using a water-soluble pH-indicator. It is shown that ChR2 released protons prior to uptake with a stoichiometry of 0.3 protons per ChR2. Comparison to the photocycle kinetics revealed that proton release and uptake match rise and decay of the View the MathML sourceP3520 intermediate. As the View the MathML sourceP3520 state also represents the conductive state of cation channeling, the concurrence of proton pumping and channel gating implies an intimate mechanistic link of the tw…

Models MolecularRhodopsinProtonKineticsBiophysicsAnalytical chemistryChannelrhodopsinBacteriorhodopsinBiochemistry530Protein Structure SecondaryProton transferStructural BiologyGeneticsMolecular BiologyIon channelMembrane potentialbiologyChemistryfungiBacteriorhodopsinBiological TransportCell BiologyHydrogen-Ion ConcentrationProton PumpsOptogeneticsKineticsRhodopsinBiophysicsbiology.proteinProtonsIon channelStoichiometry
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Photoexcitation of the P4480 State Induces a Secondary Photocycle That Potentially Desensitizes Channelrhodopsin-2

2018

Channelrhodopsins (ChRs) are light-gated cation channels. In spite of their wide use to activate neurons with light, the photocurrents of ChRs rapidly decay in intensity under both continuous illum...

0301 basic medicine010405 organic chemistryChemistryChannelrhodopsinGeneral Chemistry01 natural sciencesBiochemistryCatalysis0104 chemical sciencesPhotoexcitation03 medical and health sciences030104 developmental biologyColloid and Surface ChemistryBiophysicsJournal of the American Chemical Society
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