0000000000776115
AUTHOR
Ece Neslihan Aybeke
Study of the dynamics of biomolecules by high speed atomic force microscopy and surface enhanced Raman spectroscopy
This thesis focuses on the coupling of High–Speed Atomic Force Microscopy (HS-AFM) and Surface Enhanced Raman Spectroscopy (SERS) for biomolecule analysis. We have designed a fabrication protocol to manufacture “SERS-active” substrates. The efficacy of gold, silver and gold-silver bimetallic crystalline nanoparticle substrates were evaluated. We have investigated the impact of optical and morphological features of the substrates on Raman signal intensity by analyzing well-known samples such as bipyridine ethylene and methylene blue molecules. We took an interest in three distinct biological problematics with HS-AFM and SERS analyses. First, we have detected the chemical signature of cytochr…
A mucosal pellicle modifies the physical properties of epithelial
International audience
International Scanning Probe Microscopy Conference
International audience