6533b831fe1ef96bd12994e5

RESEARCH PRODUCT

Study of the dynamics of biomolecules by high speed atomic force microscopy and surface enhanced Raman spectroscopy

Ece Neslihan Aybeke

subject

Tip–Enhanced Raman Spectroscopy (TERS)Surface Enhanced Raman Spectroscopy (SERS)CellsProteinsDetergent resistant membrane domains (DRMs)ProtéinesSpectroscopie Raman exaltée de surface (SERS)Nanoparticle substratesNoroviruses (NoVs)Substrats de nanoparticulesHigh–Speed Atomic Force Microscopy (HS-AFM)Microscopie à force atomique haute-vitesse (HS-AFM)Diffusion Raman exaltée par effet de pointe (TERS)Détergent résistant membrane domaines (DRMs)Plasmons de Surface Localisé (LSP)Cellules[PHYS.PHYS] Physics [physics]/Physics [physics]Localized Surface Plasmons (LSP)

description

This thesis focuses on the coupling of High–Speed Atomic Force Microscopy (HS-AFM) and Surface Enhanced Raman Spectroscopy (SERS) for biomolecule analysis. We have designed a fabrication protocol to manufacture “SERS-active” substrates. The efficacy of gold, silver and gold-silver bimetallic crystalline nanoparticle substrates were evaluated. We have investigated the impact of optical and morphological features of the substrates on Raman signal intensity by analyzing well-known samples such as bipyridine ethylene and methylene blue molecules. We took an interest in three distinct biological problematics with HS-AFM and SERS analyses. First, we have detected the chemical signature of cytochrome b5 protein. This study was followed by the investigation of conformational changes of small heat shock leuconostoc oenos Lo 18 protein in function of pH level and concentrations. The last application consists to the analyse a membrane and a virus interaction. In order to realize simultaneous Raman/AFM analysis, we have adapted our fabrication protocol to cover the surface of commercial AFM probes by crystalline gold nanoparticles. Tip – Enhanced Raman Spectroscopy (TERS) studies were performed on molybdenum disulfide to evaluate the quality of TERS probes. In the last part of this work, we have designed a new setup to combine Ando’s HS-AFM setup with Raman spectroscopy. We present the modifications that have been carried out and the challenges that we have encountered.

https://theses.hal.science/tel-01250689