Evaluation of automated hematologic VCS parameters in severe sepsis and septic shock: a case-control study.

A cheap and quick hematologic diagnostic parameter for detection of sepsis would have both economic and therapeutic benefits. The Coulter LH series hematology analyzer uses the VCS technology (Volume, Conductivity, Laser Scatter) providing information about cell volume, size, internal structure, and surface morphology. Many authors analyzed the clinical usefulness of VCS parameters in reactive neutrophils for detection of sepsis. An increase in mean cell volume and a decrease in mean light scatter in septic patients have been described. Our aim is to verify the correlation between VCS parameters and sepsis.

LyghtCycler Septifast in early diagnosis of sepsis: our experience.

LightCycler SeptiFast in early diagnosis of sepsis: our experience.

The conventional sepsis diagnosis, using the cultivation technique, needs 24 hours for bacterial identification and 36 hours for fungal. The use of empiric therapy makes the growth of bacteria and fungi slower or may yield negative findings in many cases of septic shock. The molecular technique can contribute to a more rapid and specific diagnosis in septic patients. SeptiFast detects 26 bacterial and fungal species DNAs, using the PCR in real time and giving results after 6 hours. This is important for de-escalation therapy and beginning of appropriate antibiotic treatment. The aim of this study is to evaluate the sensibility and the specificity of the SeptiFast test versus traditional dia…