0000000000890385
AUTHOR
Fabienne Vienney
A thin layer-based amperometric enzyme immunoassay for the rapid and sensitive diagnosis of respiratory syncytial virus infections
Abstract A simple electrochemical sandwich immunoassay involving a polystyrene microarray slide coated with monoclonal capture antibodies and carbon screen-printed sensors (SPS) was designed for the rapid diagnosis of respiratory syncytial virus (RSV). The detection of the antibody-antigen complex formation relied on the use of a horseradish peroxidase conjugate. Its chronoamperometric measurement detection was performed by confining a droplet of H 2 O 2 /3,3',5,5'-tetramethylbenzidine enzyme substrate/mediator solution within a thin layer between one spot of the microarray and the surface of one screen-printed electrochemical cell. The accumulation of the enzyme product in the thin film of…
An electrochemical DNA biosensor for the detection of CTX-M extended-spectrum β-lactamase-producing Escherichia coli in soil samples
International audience; An electrochemical hybridization assay involving neutravidin-coated carbon screen-printed electrodes and an HRP-based detection have been shown to provide an effective tool for the genotypic analysis of extended-spectrum beta-lactamase-producing E. coli strains in complex samples such as soil. (c) 2012 Elsevier B.V. All rights reserved.
Amperometric detection of extended-spectrum β-lactamase activity : application to the characterization of resistant E.coli strains
EA MERS CT3; International audience; The amperometric detection of extended-spectrum β-lactamase (ESBL) with carbon screen-printed sensors was investigated in the presence of the Nitrocefin, a commercially-available β-lactamase chromogenic cephalosporin substrate. Using an ESBL isolated from a clinical sample, it was shown for the first time that the intensity of a specific anodic pic current (EP = [similar]+0.3 V vs. Ag/AgCl) resulting from the catalytic hydrolysis of the β-lactam ring was proportional to the amount of ESBL. The proof-of-principle of a novel susceptibility assay for the rapid and accurate identification of ESBL- producing bacteria was then demonstrated. The detection schem…
Le « Semestre Rebond » après la réorientation obligatoire en première année commune des études de santé (PACES) en France
Contexte et problématique :L’échec en premier cycle universitaire s’est constitué en question politique et scientifique, avec une situation encore plus marquée pour les études de santé. Pourtant, peu de recherches encore sont consacrées à ces situations et aux dispositifs d’aide proposés.Objectifs :Cette recherche vise à caractériser les profils des étudiants en situation d’échec, leurs stratégies de réorientation au nombre desquelles l’accès à un dispositif d’accompagnement proposé à l’université de Bourgogne, le semestre Rebond. Elle met en évidence les apports de ce dispositif perçus par les étudiants et ses effets sur les parcours de formation ultérieurs.Méthodes :L’étude mobilise les d…