0000000000890385

AUTHOR

Fabienne Vienney

showing 4 related works from this author

A thin layer-based amperometric enzyme immunoassay for the rapid and sensitive diagnosis of respiratory syncytial virus infections

2012

Abstract A simple electrochemical sandwich immunoassay involving a polystyrene microarray slide coated with monoclonal capture antibodies and carbon screen-printed sensors (SPS) was designed for the rapid diagnosis of respiratory syncytial virus (RSV). The detection of the antibody-antigen complex formation relied on the use of a horseradish peroxidase conjugate. Its chronoamperometric measurement detection was performed by confining a droplet of H 2 O 2 /3,3',5,5'-tetramethylbenzidine enzyme substrate/mediator solution within a thin layer between one spot of the microarray and the surface of one screen-printed electrochemical cell. The accumulation of the enzyme product in the thin film of…

Time Factorsrespiratory syncytial virus[SDV]Life Sciences [q-bio]Biosensing TechniquesRespiratory Syncytial Virus Infectionsscreen-printed sensorSensitivity and SpecificityHorseradish peroxidaseVirusAnalytical ChemistryImmunoenzyme TechniquesElectrochemistrymedicineHumans[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyimmunoassayAntigens ViralHorseradish PeroxidaseChromatographybiologymedicine.diagnostic_testChemistryAntibodies MonoclonalRespiratory infectionSubstrate (chemistry)Molecular biologyAmperometryRespiratory Syncytial VirusesHRP labelImmunoassay[SDE]Environmental Sciencesbiology.proteinColorimetrythin layer amperometric detectionAntibodyConjugate
researchProduct

An electrochemical DNA biosensor for the detection of CTX-M extended-spectrum β-lactamase-producing Escherichia coli in soil samples

2013

International audience; An electrochemical hybridization assay involving neutravidin-coated carbon screen-printed electrodes and an HRP-based detection have been shown to provide an effective tool for the genotypic analysis of extended-spectrum beta-lactamase-producing E. coli strains in complex samples such as soil. (c) 2012 Elsevier B.V. All rights reserved.

DNA BacterialMicrobiology (medical)Soil test[SDV]Life Sciences [q-bio]Biosensing TechniquesBiologymedicine.disease_causeElectrochemistry01 natural sciencesMicrobiologybeta-LactamasesMicrobiologySoil03 medical and health scienceschemistry.chemical_compoundEscherichia colimedicine[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyCTX-MScreen-printed carbon electrodesMolecular BiologyEscherichia coliSoil Microbiology0303 health sciencesChromatographyIDENTIFICATION030306 microbiology010401 analytical chemistryE. coliElectrochemical TechniquesExtended-spectrum beta-lactamase0104 chemical scienceschemistry[SDE]Environmental SciencesBiosensorSoil microbiologyDNA biosensorDNAJournal of Microbiological Methods
researchProduct

Amperometric detection of extended-spectrum β-lactamase activity : application to the characterization of resistant E.coli strains

2015

EA MERS CT3; International audience; The amperometric detection of extended-spectrum β-lactamase (ESBL) with carbon screen-printed sensors was investigated in the presence of the Nitrocefin, a commercially-available β-lactamase chromogenic cephalosporin substrate. Using an ESBL isolated from a clinical sample, it was shown for the first time that the intensity of a specific anodic pic current (EP = [similar]+0.3 V vs. Ag/AgCl) resulting from the catalytic hydrolysis of the β-lactam ring was proportional to the amount of ESBL. The proof-of-principle of a novel susceptibility assay for the rapid and accurate identification of ESBL- producing bacteria was then demonstrated. The detection schem…

Cefotaximemedicine.drug_classélectrochimie[SDV]Life Sciences [q-bio]CephalosporinAnalytical chemistryBiochemistrybeta-LactamasesAnalytical Chemistry03 medical and health sciencesClavulanic acidDrug Resistance BacterialElectrochemistrymedicineEscherichia colipolycyclic compoundsEnvironmental ChemistryNitrocefin[SDV.BV]Life Sciences [q-bio]/Vegetal Biologyélectrode sérigraphiéeSpectroscopybêta-lactamase à spectre étendu (BLSE)Enzyme Assays030304 developmental biologyDetection limit0303 health sciencesChromatographybiology030306 microbiologyChemistryChromogenicbactériologienitrocéfineHydrolysisbiochemical phenomena metabolism and nutritionbiology.organism_classificationbacterial infections and mycosesAmperometryAnti-Bacterial AgentsCephalosporinscultureampérométrie[SDE]Environmental SciencesBacteriamedicine.drugbactériologie;culture;Escherichia coli;bêta-lactamase à spectre étendu (BLSE);électrochimie;ampérométrie;électrode sérigraphiée;nitrocéfine
researchProduct

Le « Semestre Rebond » après la réorientation obligatoire en première année commune des études de santé (PACES) en France

2018

Contexte et problématique :L’échec en premier cycle universitaire s’est constitué en question politique et scientifique, avec une situation encore plus marquée pour les études de santé. Pourtant, peu de recherches encore sont consacrées à ces situations et aux dispositifs d’aide proposés.Objectifs :Cette recherche vise à caractériser les profils des étudiants en situation d’échec, leurs stratégies de réorientation au nombre desquelles l’accès à un dispositif d’accompagnement proposé à l’université de Bourgogne, le semestre Rebond. Elle met en évidence les apports de ce dispositif perçus par les étudiants et ses effets sur les parcours de formation ultérieurs.Méthodes :L’étude mobilise les d…

020205 medical informatics05 social sciences0202 electrical engineering electronic engineering information engineering050301 education02 engineering and technology0503 educationPédagogie Médicale
researchProduct