6533b857fe1ef96bd12b46fb

RESEARCH PRODUCT

An electrochemical DNA biosensor for the detection of CTX-M extended-spectrum β-lactamase-producing Escherichia coli in soil samples

Murielle RocheletAudrey MembrillaFabienne VienneySébastien SolanasAlain Hartmann

subject

DNA BacterialMicrobiology (medical)Soil test[SDV]Life Sciences [q-bio]Biosensing TechniquesBiologymedicine.disease_causeElectrochemistry01 natural sciencesMicrobiologybeta-LactamasesMicrobiologySoil03 medical and health scienceschemistry.chemical_compoundEscherichia colimedicine[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyCTX-MScreen-printed carbon electrodesMolecular BiologyEscherichia coliSoil Microbiology0303 health sciencesChromatographyIDENTIFICATION030306 microbiology010401 analytical chemistryE. coliElectrochemical TechniquesExtended-spectrum beta-lactamase0104 chemical scienceschemistry[SDE]Environmental SciencesBiosensorSoil microbiologyDNA biosensorDNA

description

International audience; An electrochemical hybridization assay involving neutravidin-coated carbon screen-printed electrodes and an HRP-based detection have been shown to provide an effective tool for the genotypic analysis of extended-spectrum beta-lactamase-producing E. coli strains in complex samples such as soil. (c) 2012 Elsevier B.V. All rights reserved.

https://doi.org/10.1016/j.mimet.2012.11.019