0000000000890387
AUTHOR
Sébastien Solanas
showing 4 related works from this author
A thin layer-based amperometric enzyme immunoassay for the rapid and sensitive diagnosis of respiratory syncytial virus infections
2012
Abstract A simple electrochemical sandwich immunoassay involving a polystyrene microarray slide coated with monoclonal capture antibodies and carbon screen-printed sensors (SPS) was designed for the rapid diagnosis of respiratory syncytial virus (RSV). The detection of the antibody-antigen complex formation relied on the use of a horseradish peroxidase conjugate. Its chronoamperometric measurement detection was performed by confining a droplet of H 2 O 2 /3,3',5,5'-tetramethylbenzidine enzyme substrate/mediator solution within a thin layer between one spot of the microarray and the surface of one screen-printed electrochemical cell. The accumulation of the enzyme product in the thin film of…
An electrochemical DNA biosensor for the detection of CTX-M extended-spectrum β-lactamase-producing Escherichia coli in soil samples
2013
International audience; An electrochemical hybridization assay involving neutravidin-coated carbon screen-printed electrodes and an HRP-based detection have been shown to provide an effective tool for the genotypic analysis of extended-spectrum beta-lactamase-producing E. coli strains in complex samples such as soil. (c) 2012 Elsevier B.V. All rights reserved.
Amperometric detection of extended-spectrum β-lactamase activity : application to the characterization of resistant E.coli strains
2015
EA MERS CT3; International audience; The amperometric detection of extended-spectrum β-lactamase (ESBL) with carbon screen-printed sensors was investigated in the presence of the Nitrocefin, a commercially-available β-lactamase chromogenic cephalosporin substrate. Using an ESBL isolated from a clinical sample, it was shown for the first time that the intensity of a specific anodic pic current (EP = [similar]+0.3 V vs. Ag/AgCl) resulting from the catalytic hydrolysis of the β-lactam ring was proportional to the amount of ESBL. The proof-of-principle of a novel susceptibility assay for the rapid and accurate identification of ESBL- producing bacteria was then demonstrated. The detection schem…
Long Term Exposure of Agricultural Soil to Veterinary Antibiotics Changes the Population Structure of Symbiotic Nitrogen-Fixing Bacteria Occupying No…
2017
International audience; Antibiotics can be entrained onto agricultural land through the application of animal manures, human biosolids, or recycled wastewater for irrigation. In order to evaluate the impacts on soil microorganisms of exposure to antibiotics, a series of replicated plots were initiated in 1999 at the AAFC research farm in London ON. Every spring a mixture of sulfamethazine, chlortetracycline and tylosin is incorporated directly into the soil to attain concentrations ranging from 0.1 to 10 mg/kg, and these are then seeded with soybeans. In the present study nodules were isolated from soybean plants growing in antibiotic treated and control soils in 2012, after 14 annual treat…