IMMUNOHISTOCHEMICAL DISTRIBUTION OF CONSTITUTIVE NOS ISOFORMS IN PROSTATE, SEMINAL VESICLES AND BULBOURETHRAL GLANDS IN SOME MAMMALS MALE GENITAL TRACT

Association Between Interleukin-10 Polymorphisms and Alzheimer's Disease: A Systematic Review and Meta-Analysis

UNLABELLED It has been hypothesized that polymorphisms of interleukin (IL)-10 genes affect the risk of developing late onset Alzheimer's disease (AD). However, results of different studies are often inconsistent. Our aim was to investigate by meta-analysis the association of the common polymorphisms comprehensively defining the genetic variability of the IL-10 gene with AD risk. Fifteen studies investigating the association between IL-10 polymorphisms (-1082, -819, -592) and AD were found and analyzed. The model-free approach was applied to meta-analyze these case-control genetic association studies. Available data suggested an association between -1082 polymorphism and AD risk with a margi…

Immunohistochemical expression of Cytokeratins in periapical lesions

Objective. This study seeks to clarify the histological origin of apical lesions, through the expression of certain cytokeratins.Study design. In 3 years 30 patients were selected. After clinical and radiological exams were chosen patients who had severe apical lesions and tooth decay with exposure of the pulp chamber left untreated for very long periods (more the 12 months). Serial slides were prepared both for immunohistochemistry and Hematoxylin- Eosin. Material and methods. Twenty specimens coming from the 30 patients were used for our purpose. All periapical lesions were surgically extracted and fixed in Bouin mixture, and embedded in paraffin. Samples were processed immunohistochemica…

Effect of chronic treatment with CB1 receptor antagonist (Rimonabant) on B-cells apoptosis in the pancreas of obese Zucker rats. An immunohistochemical study

HSP10,HSP70 AND HSP90 IMMUNOHISTOCHEMICAL LEVELS CHANGE IN ULCERATIVE COLITIS AFTER THERAPY

Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD) characterized by damage of large bowel mucosa and frequent extra-intestinal autoimmune comorbidities. The role played in IBD pathogenesis by molecular chaperones known to interact with components of the immune system involved in inflammation is unclear. We previously demonstrated that mucosal Hsp60 decreases in UC patients treated with conventional therapies (mesalazine, probiotics), suggesting that this chaperonin could be a reliable biomarker useful for monitoring response to treatment, and that it might play a role in pathogenesis. In the present work we investigated three other heat shock protein/molecular chaperones:…

Immunohistochemical expression and apoptotic role of Caspase9 in aortic aneurysms

Immunhistochemical expression of some neuropeptides and CB1 cannabinoid receptor in rat lung. Note II: Trachea and Bronchi

IMMUNOHISTOCHEMICAL DISTRIBUTION OF ORPHANIN FQ IN THE RAT GASTROINTESTINAL APPARATUS AND PANCREAS

Histopathology of Skeletal Muscle in a Distal Motor Neuropathy Associated with a Mutant CCT5 Subunit: Clues for Future Developments to Improve Differential Diagnosis and Personalized Therapy

Genetic chaperonopathies are rare but, because of misdiagnosis, there are probably more cases than those that are recorded in the literature and databases. This occurs because practitioners are generally unaware of the existence and/or the symptoms and signs of chaperonopathies. It is necessary to educate the medical community about these diseases and, with research, to unveil their mechanisms. The structure and functions of various chaperones in vitro have been studied, but information on the impact of mutant chaperones in humans, in vivo, is scarce. Here, we present a succinct review of the most salient abnormalities of skeletal muscle, based on our earlier report of a patient who carried…

Natural autofluorescence in archeological bone

The human bone present a natural autofluorescence which is tipically induced by natural antibiotiscs absorbed by collagen and in part is due to phenomena not weel know and referred to an enigmatic endogenous fluophore. Is iintristing for us to see the autofluorescence in the various age of the observed samples.

Immunohistochemical expression of iNOS and eNOS in the venous ulcers related to chronic venous insufficiency (CVI).

Comparative analysis of Hsp10 and Hsp90 in large bowel healthy mucosa and adenocarcinomas

Heat shock proteins (Hsps) are an important class of molecules with various functions. Their classic role is to assist other proteins in folding and re-folding and, when proteins are defective or irreversibly misfolded, to drive their degradation. For this reason, some Hsps are also named molecular chaperones. During evolution, this class of proteins has also acquired extrachaperoning roles such as participation in immune system regulation, cell differentiation, programmed cell death and carcinogenesis. Hsp10 is a partner of Hsp60 in the Hsp60/10 folding machine, but numerous scientific studies have shown that Hsp10 may also play other roles. In fact, Hsp10 seems to have an immunomodulatory…

Il controllo nitrossidergico e peptidergico della secrezione salivare nella parotide di alcuni mammiferi

NO and Chronic Venous Insufficiency (CVI): Immunohistochemical Evaluation of iNOS and eNOS Isofroms in the Venous Ulcers. Clinical and Therapeutical Possible Implications

Does the orthodontic treatment induces dental pulp cellular death? Caspase-3 and-9, HSP60 and TUNEL expression

Objective: To evaluate the hypothesis of human dental pulp cell death during orthodontic treatement (O.T) and the degree of apoptosis through the expression level of the proteins Caspase-3,-9, TUNEL and HSP60. Materials and methods: Human dental Pulp were coming from both male and female patients (N=20; age 10-14years). The technique used was the Straight Wire, which involves Nickel-Titanium or Steel archwires. The increase of pressure applied on teeth was gradual. Some patients were subjected to a premolar extraction after 3 months treatment, and others after 6 months. Samples were Bouin-fixed, paraffin-embedded and afterwards processed for immunohistochemistry using anti-caspase-3,-9 anti…

Immunohistochemical Expression of NO-synthase Constitutive Isoform (cNOS)in Mammals Normal Prostate and in Human Prostatic Obstructive Hyperplasia

The aim of present study is to investigate the immunohistochemical identification of cNOS-containing prostatic structures in various species and in hyperplastic human prostate. We used specimens of rat, guinea pig and hamster. Human prostatic specimens were removed from patients suffering of prostatic hyperplasia. The study was processed by ABC method. Immunohistochemical staining with anti nNOS antibody revealed the existence of strong reactivity in numerous ganglionic cell bodies and varicose nerve fibers in the fibromuscular stroma, in smooth cells around acini and in blood vessels endothelium. Surprisingly nNOS immunoreactivity appears in the glandular epithelium. In the hyperplastic pr…

Nandrolone decanoate interferes on testosterone biosynthesis and alters blood-testis barrier.

Nandrolone decanoate (ND) is a synthetic testosterone analogue considered one of the most commonly abused anabolic androgenic steroids by adolescents and athletes. ND is alleged to promote an increase in muscle mass and improves both physical appearance and sporting performance, but ND abuse is often associated with serious adverse effects, interfering with the endocrine system and the reproductive system. In a previous study, we demonstrated that ND treatment of Leydig cells interferes with the biosynthesis of testosterone in a dose increase-dependent fashion (1). As a consequence of the results obtained in vitro, in this study an animal model was utilized to better understand the side eff…

Immunohistochemical expression of CB1 cannabinoid receptor in the laryngeal-tracheal tract in humans suffering from laryngeal neoplasia

Immunhistochemical studies on leptin, ghrelin, orexin and orphanin fq expression in the stomach of obese (fa/fa)zucker rats

Immunohistochemical expression of iNOS and metalloproteinases MMP2 and MMP9 in abdominal inflammatory aortic Aneurysms

Immunohistochemical expression of CB1 cannabinoids receptor in rat lung

The Bio-Molecular Dynamics of Dental Pulp in Different Clinical Scenarios

Dental pulp (DP) is a very dynamic tissue both in health and in disease. When exposed to stressors and pathological conditions. It undergoes a complex series of biological reactions whereby alterations affect the pulp tissue at tissue cellular and molecular levels. The aim of this review is to update the reader on the various bio-molecular alterations in the dental pulp under different clinical conditions: orthodontic treatment (OT), caries, pulpitis and others. The morphological changes in the composition of the DP rang from the reversible remodeling to apoptosis and sometimes necrosis. Many apoptotic factors are involved like Bcl2, Bax and the significant increase in Caspases 9 and 3, as …

The role of new neurocrine and neuroendocrine messengers on the regulation of the function of major salivary glands in some mammals. An immunohistochemical study

Heat-shock protein 60 kDa and atherogenic dyslipidemia in patients with untreated mild periodontitis: a pilot study

Identification of predictors of cardiovascular risk can help in the prevention of pathologic episodes and the management of patients at all stages of illness. Here, we investigated the relationships between serum levels of Hsp60 and dyslipidemia in patients with periodontitis by performing a cross-sectional study of 22 patients with mild periodontitis without any prior treatment for it (i.e., drug naive) and 22 healthy controls, matched for age and body mass index (BMI). All subjects were evaluated for periodontal status, gingival inflammation, and oral hygiene. Levels of circulating Hsp60, C-reactive protein (CRP), and plasma lipids were measured, and small, dense low-density lipoproteins …

Probiotics Can Cure Oral Aphthous-Like Ulcers in Inflammatory Bowel Disease Patients: A Review of the Literature and a Working Hypothesis

Dysbiosis has been associated with the onset of several chronic autoimmune or inflammatory pathologies (e.g., inflammatory bowel diseases—IBD), because of its primary role in the establishment of a chronic inflammatory process leading to tissue damage. Inflammatory bowel diseases can even involve areas far away from the gut, such as the extraintestinal manifestations involving the oral cavity with the onset of aphthous-like ulcers (ALU). Studies carried out on animal models have shown that intestinal dysbiosis may be related to the development of autoimmune diseases, even if the mechanisms involved are not yet well known. The aim of this paper is to verify the hypothesis that in inflammator…

Immunohistochemical expression of Orphanin FQ in the rat male genital tract

Immunohistochemical distribution of NOS Isoform in the Embryofoetal structures of Human encephalon

Nadph Diaphorase Histochemical Presence in some Mammal's Penis

Intrabdominal and extrabdominal portion of penis (rat, guinea pig, hamster and rabbit) have been used for histochemical activity Nadph-diaphorase. Pharmacological and electrophysiological studies, both in vivo and in vitro, make us to belive that, in man, dog, rabbit and rat, the relaxation of vascular smooth muscle of the cavernous body of penis, that is responsible for erection, is produced by the activation of a Non-Adrenergic, Non-Cholinergic (NANC) nervous pathway whose mediator is a new neurotrasmitter: Nitric Oxide (NO). Our observations provide the morphohistochemical base for the localization of NADPH-diaphorase in the different constitutive structures of the penis in some mammalia…

Immunohistochemical evidence for the presence of CB1 cannabinoid receptor in rat salivery glands

Emerging Immnunohistochemical evidence for Direct Peripheral Control of Endocannabinoids on the Gastrointestinal Tract and Pancreas of Obese (fa/fa)and Lean Zucker Rats (Pathophysiological Implications)

This research has the objective to investigate immunohistochemical expression of CB1 receptor and its probable changes in Gastroenteropancreatic system (GEP) of obese and lean Zucker rats and understand the endocannabinoid pathophysiological implications in the obesity. Male obese (fa/fa) and lean Zucker rats 6 weeks old were obtained from Harlan Italy Srl; the rats were sacrificed at 8, 12 and 16 weeks old. Normal rats also were sacrificed. Specimens of stomach, jejunum-ileum and pancreas were fixed in Bouin’s mixture and embedded in paraffin; obtained sections were processed with anti-CB1 (Biosource Europe SA) by Streptavidin-Biotin-Complex Method. The findings show that CB1 receptor is e…

Hsp60 as a Novel Target in IBD Management: A Prospect

Inflammatory bowel disease (IBD) encompasses various pathological conditions similar but distinct that share a multifactorial etiology, including involvement of the intestinal barrier function, the immune system, and intestinal microorganisms. Hsp60 is a chaperonin component of the chaperoning system, present in all cells and tissues, including the intestine. It plays important roles in cell physiology outside and inside mitochondria, its canonical place of residence. However, Hsp60 can also be pathogenic in many conditions, the Hsp60 chaperonopathies, possibly including IBD. The various clinico-pathological types of IBD have a complicated mix of causative factors, among which Hsp60 can be …

Immunohistochemical expression of Orexin A and Orexin Type 2 receptors in the rat gastrointestinal apparatus and endocrine pancreas

RILIEVI IMMNOISTOCHIMICI DELLA ORFANINA FQ NELL'APPARATO GENITO URINARIO DEL RATTO (IMMUNOHISTOCHEMICAL STUDY OF ORPHANIN FQ IN RAT'S UROGENITAL TRACT)

Nociceptin/Orphanin FQ (OFQ) is a recently discovered endogenous neuropeptide that structurally resembles on opioid peptide. In the central nervous system this peptide is implicated in the nociception. More recently its documented distribution in other peripheral structures suggests a possible role for OFQ in regulation of other physiological functions. On this subject the mouse vas deferens is proposed as available bioassay for nociceptin. It therefore seems interesting to evaluate immunohistochemical expression of this neuropeptide in distinct segments of rat male urogenital tract. The results obtained supply the morphohistochemical basis to demonstrate that Orphanin plays a neurocrine co…

Immunohistochemical expression and distribution of orexin, orphanin and leptin in the major salivary glands of some mammals

Abstract: The aim of the study was to assess the involvement of apoptotic factors, cytokeratins and metalloproteinase- 9 in the histogenesis of both Epithelialized Gingival Lesions (EGL) and Periapical Lesions (PAL). 55 consecutive patients, 30 with PAL and 25 with EGL, were selected for the study after clinical and radiological examinations. The PAL patients had severe periapical lesions and tooth decay with exposure of the pulp chamber. All PAL and EGL biopsies were surgically extracted, fixed in 10% buffered formalin, and processed for routine light microscopy. Ten biopsies of each category were processed for immunohistochemistry (IHC). Serial paraffin sections were stained by IHC with a…

Hsp60 and human aging: Les liaisons dangereuses 

Stressors can cause abnormal intracellular accumulation of Hsp60 and its localization in extramitochondrial sites, secretion, and circulation, with immune system activation. Dysfunction of chaperones associated with their quantitative and qualitative decline with aging (chaperonopathies of aging) characterizes senescence and is a potential causal factor in the physiological deterioration that occurs with it. The role of Hsp60 in aging is not easy to elucidate, because aging is accompanied by pathologies (e.g., cardiovascular and neurodegenerative disorders, osteoporosis, diabetes, cancer, etc.) in which Hsp60 has been implicated but, although those disorders are more frequent in the elderly…

An immunohistochemical study on the cb1 receptor expression in rat salivary glands