0000000000933211
AUTHOR
Donato Cascio
showing 48 related works from this author
HEp-2 Intensity Classification based on Deep Fine-tuning
2020
Automatic Segmentation of HEp-2 Cells Based on Active Contours Model
2018
In the past years, a great deal of effort was put into research regarding Indirect Immunofluorescence techniques with the aim of development of CAD systems. In this work a method for segmenting HEp-2 cells in IIF images is presented. Such task is one of the most challenging of automated IIF analysis, because the segmentation algorithm has to cope with a large heterogeneity of shapes and textures. In order to address this problem, numerous techniques and their combinations were evaluated, in a process aimed at maximizing the figure of merit. The proposed method, for a greater definition of cellular contours, uses the active contours in the last phase of the process. The initial conditions, c…
An Automatic HEp-2 Specimen Analysis System Based on an Active Contours Model and an SVM Classification
2019
The antinuclear antibody (ANA) test is widely used for screening, diagnosing, and monitoring of autoimmune diseases. The most common methods to determine ANA are indirect immunofluorescence (IIF), performed by human epithelial type 2 (HEp-2) cells, as substrate antigen. The evaluation of ANA consist an analysis of fluorescence intensity and staining patterns. This paper presents a complete and fully automatic system able to characterize IIF images. The fluorescence intensity classification was obtained by performing an image preprocessing phase and implementing a Support Vector Machines (SVM) classifier. The cells identification problem has been addressed by developing a flexible segmentati…
Mammographic images segmentation based on chaotic map clustering algorithm
2013
Background: This work investigates the applicability of a novel clustering approach to the segmentation of mammographic digital images. The chaotic map clustering algorithm is used to group together similar subsets of image pixels resulting in a medically meaningful partition of the mammography. Methods: The image is divided into pixels subsets characterized by a set of conveniently chosen features and each of the corresponding points in the feature space is associated to a map. A mutual coupling strength between the maps depending on the associated distance between feature space points is subsequently introduced. On the system of maps, the simulated evolution through chaotic dynamics leads…
Deep CNN for IIF Images Classification in Autoimmune Diagnostics
2019
The diagnosis and monitoring of autoimmune diseases are very important problem in medicine. The most used test for this purpose is the antinuclear antibody (ANA) test. An indirect immunofluorescence (IIF) test performed by Human Epithelial type 2 (HEp-2) cells as substrate antigen is the most common methods to determine ANA. In this paper we present an automatic HEp-2 specimen system based on a convolutional neural network method able to classify IIF images. The system consists of a module for features extraction based on a pre-trained AlexNet network and a classification phase for the cell-pattern association using six support vector machines and a k-nearest neighbors classifier. The class…
A maChine and deep Learning Approach to predict pulmoNary hyperteNsIon in newbornS with congenital diaphragmatic Hernia (CLANNISH): Protocol for a re…
2021
Introduction Outcome predictions of patients with congenital diaphragmatic hernia (CDH) still have some limitations in the prenatal estimate of postnatal pulmonary hypertension (PH). We propose applying Machine Learning (ML), and Deep Learning (DL) approaches to fetuses and newborns with CDH to develop forecasting models in prenatal epoch, based on the integrated analysis of clinical data, to provide neonatal PH as the first outcome and, possibly: favorable response to fetal endoscopic tracheal occlusion (FETO), need for Extracorporeal Membrane Oxygenation (ECMO), survival to ECMO, and death. Moreover, we plan to produce a (semi)automatic fetus lung segmentation system in Magnetic Resonanc…
Unsupervised clustering method for pattern recognition in IIF images
2017
Autoimmune diseases are a family of more than 80 chronic, and often disabling, illnesses that develop when underlying defects in the immune system lead the body to attack its own organs, tissues, and cells. Diagnosis of autoimmune pathologies is based on research and identification of antinuclear antibodies (ANA) through indirect immunofluorescence (IIF) method and is performed by analyzing patterns and fluorescence intensity. We propose here a method to automatically classify the centromere pattern based on the grouping of centromeres on the cells through a clustering K-means algorithm. The described method was tested on a public database (MIVIA). The results of the test showed an Accuracy…
A multi-process system for HEp-2 cells classification based on SVM
2016
An automatic system for pre-segmented IIF images analysis was developed.A non-standard pipeline for supervised image classification was adopted.The system uses a two-level pyramid to retain some spatial information.From each cell image 216 features are extracted.15 SVM classifiers one-against-one have been implemented. This study addresses the classification problem of the HEp-2 cells using indirect immunofluorescence (IIF) image analysis, which can indicate the presence of autoimmune diseases by finding antibodies in the patient serum. Recently, studies have shown that it is possible to identify the cell patterns using IIF image analysis and machine learning techniques. In this paper we de…
Deep Convolutional Neural Network for HEp-2 fluorescence intensity classification
2019
Indirect ImmunoFluorescence (IIF) assays are recommended as the gold standard method for detection of antinuclear antibodies (ANAs), which are of considerable importance in the diagnosis of autoimmune diseases. Fluorescence intensity analysis is very often complex, and depending on the capabilities of the operator, the association with incorrect classes is statistically easy. In this paper, we present a Convolutional Neural Network (CNN) system to classify positive/negative fluorescence intensity of HEp-2 IIF images, which is important for autoimmune diseases diagnosis. The method uses the best known pre-trained CNNs to extract features and a support vector machine (SVM) classifier for the …
A Fuzzy Logic C-Means Clustering Algorithm to Enhance Microcalcifications Clusters in Digital Mammograms
2011
The detection of microcalcifications is a hard task, since they are quite small and often poorly contrasted against the background of images. The Computer Aided Detection (CAD) systems could be very useful for breast cancer control. In this paper, we report a method to enhance microcalcifications cluster in digital mammograms. A Fuzzy Logic clustering algorithm with a set of features is used for clustering microcalcifications. The method described was tested on simulated clusters of microcalcifications, so that the location of the cluster within the breast and the exact number of microcalcifications is known.
A completely automated CAD system for mass detection in a large mammographic database
2006
Mass localization plays a crucial role in computer-aided detection (CAD) systems for the classification of suspicious regions in mammograms. In this article we present a completely automated classification system for the detection of masses in digitized mammographic images. The tool system we discuss consists in three processing levels: (a) Image segmentation for the localization of regions of interest (ROIs). This step relies on an iterative dynamical threshold algorithm able to select iso-intensity closed contours around gray level maxima of the mammogram. (b) ROI characterization by means of textural features computed from the gray tone spatial dependence matrix (GTSDM), containing secon…
Computer-Assisted Classification Patterns in Autoimmune Diagnostics: The AIDA Project.
2016
International audience; Antinuclear antibodies (ANAs) are significant biomarkers in the diagnosis of autoimmune diseases in humans, done by mean of Indirect ImmunoFluorescence (IIF) method, and performed by analyzing patterns and fluorescence intensity. This paper introduces the AIDA Project (autoimmunity: diagnosis assisted by computer) developed in the framework of an Italy-Tunisia cross-border cooperation and its preliminary results. A database of interpreted IIF images is being collected through the exchange of images and double reporting and a Gold Standard database, containing around 1000 double reported images, has been settled. The Gold Standard database is used for optimization of …
A REST-based framework to support non-invasive and early coeliac disease diagnosis
2019
The health sector has traditionally been one of the early adopters of databases, from the most simple Electronic Health Record (formerly Computer-Based Patient Record) systems in use in general practice, hospitals and intensive care units to big data, multidata based systems used to support diagnosis and care decisions. In this paper we present a framework to support non-invasive and early diagnosis of coeliac disease. The proposed framework makes use of well-known technologies and techniques, both hardware and software, put together in a novel way. The main goals of our framework are: (1) providing users with a reliable and fast repository of a large amount of data; (2) to make such reposi…
Energy Recovery of Multiple Charge Sharing Events in Room Temperature Semiconductor Pixel Detectors
2021
Multiple coincidence events from charge-sharing and fluorescent cross-talk are typical drawbacks in room-temperature semiconductor pixel detectors. The mitigation of these distortions in the measured energy spectra, using charge-sharing discrimination (CSD) and charge-sharing addition (CSA) techniques, is always a trade-off between counting efficiency and energy resolution. The energy recovery of multiple coincidence events is still challenging due to the presence of charge losses after CSA. In this work, we will present original techniques able to correct charge losses after CSA even when multiple pixels are involved. Sub-millimeter cadmium–zinc–telluride (CdZnTe or CZT) pixel detectors we…
A fourier-based algorithm for micro-calcification enhancement in mammographic images
2008
Breast cancer is the most widespread cancer in women in the world; it manifests mostly in two forms: microcalcifications and massive lesions. These two forms differ in density, size, shape and number. Consequently, there are two different kinds of mammographic CAD algorithms: those for microcalcifications detection, and those for massive lesions detection. The microcalcifications detection is a hard task, since they are quite small and often poorly contrasted against the background, especially in images affected by digitization noise. In a CAD system the ROI Hunter plays an important role, because missed microcalcifications at this level are definitely lost. For this reason, highlighting me…
Mammogram Segmentation by Contour Searching and Mass Lesions Classification with Neural Network
2006
The mammography is the most effective procedure for an early diagnosis of the breast cancer. In this paper, an algorithm for detecting masses in mammographic images will be presented. The database consists of 3762 digital images acquired in several hospitals belonging to the MAGIC-5 collaboration (Medical Applications on a Grid Infrastructure Connection). A reduction of the whole image's area under investigation is achieved through a segmentation process, by means of a ROI Hunter algorithm, without loss of meaningful information. In the following classification step, feature extraction plays a fundamental role: some features give geometrical information, other ones provide shape parameters.…
A method to reduce the FP/imm number through CC and MLO views comparison in mammographic images
2008
In this paper we propose a method to reduce the FP/imm number through CC and MLO mammographic views comparison of the same patient. The proposed solution uses the symmetry properties of the breast to compute a geometric transformation that permits to represent the two images in comparable coordinates systems. Through this method, potential pathological ROIs of one of the projections are correlated with the ROIs in the second view. To show the effectiveness of the result we apply the method on a dataset composed of 112 couples of pathological images. Experiments shows that method enables a reduction by up to 700/0 of the FP/imm number detected after the classification step
Computer assisted diagnosis (CAD) in mammography. Comparison of diagnostic accuracy of a new algorithm (Cyclopus(R), Medicad) with two commercial sys…
2008
The study compares the diagnostic accuracy (correct identification of cancer) of a new computer-assisted diagnosis (CAD) system (Cyclopus) with two other commercial systems (R2 and CADx). Cyclopus was tested on a set of 120 mammograms on which the two compared commercial systems had been previously tested. The set consisted of mammograms reported as negative, preceding 31 interval cancers reviewed as screening error or minimal sign, and of 89 verified negative controls randomly selected from the same screening database. Cyclopus sensitivity was 74.1% (R2=54.8%; CADx=41.9%) and was higher for interval cancers reviewed as screening error (90.9%; R2=54.5%; CADx=81.8%) compared with those revie…
Room-Temperature X-ray response of cadmium-zinc-Telluride pixel detectors grown by the vertical Bridgman technique
2020
In this work, the spectroscopic performances of new cadmium–zinc–telluride (CZT) pixel detectors recently developed at IMEM-CNR of Parma (Italy) are presented. Sub-millimetre arrays with pixel pitch less than 500 µm, based on boron oxide encapsulated vertical Bridgman grown CZT crystals, were fabricated. Excellent room-temperature performance characterizes the detectors even at high-bias-voltage operation (9000 V cm−1), with energy resolutions (FWHM) of 4% (0.9 keV), 1.7% (1 keV) and 1.3% (1.6 keV) at 22.1, 59.5 and 122.1 keV, respectively. Charge-sharing investigations were performed with both uncollimated and collimated synchrotron X-ray beams with particular attention to the mitigation o…
Performance of Fine-Tuning Convolutional Neural Networks for HEp-2 Image Classification
2020
The search for anti-nucleus antibodies (ANA) represents a fundamental step in the diagnosis of autoimmune diseases. The test considered the gold standard for ANA research is indirect immunofluorescence (IIF). The best substrate for ANA detection is provided by Human Epithelial type 2 (HEp-2) cells. The first phase of HEp-2 type image analysis involves the classification of fluorescence intensity in the positive/negative classes. However, the analysis of IIF images is difficult to perform and particularly dependent on the experience of the immunologist. For this reason, the interest of the scientific community in finding relevant technological solutions to the problem has been high. Deep lea…
Automated approach for indirect immunofluorescence images classification based on unsupervised clustering method
2018
Autoimmune diseases (ADs) are a collection of many complex disorders of unknown aetiology resulting in immune responses to self-antigens and are thought to result from interactions between genetic and environmental factors. ADs collectively are amongst the most prevalent diseases in the U.S., affecting at least 7% of the population. The diagnosis of ADs is very complex, the standard screening methods provides seeking and recognizing of Antinuclear Antibodies (ANA) by Indirect ImmunoFluorescence (IIF) based on HEp-2 cells. In this paper an automatic system able to identify and classify the Centromere pattern is presented. The method is based on the grouping of centromeres present on the cell…
Recognizing the Emergent and Submerged Iceberg of the Celiac Disease: ITAMA Project-Global Strategy Protocol.
2022
Coeliac disease (CD) is frequently underdiagnosed with a consequent heavy burden in terms of morbidity and health care costs. Diagnosis of CD is based on the evaluation of symptoms and anti-transglutaminase antibodies IgA (TGA-IgA) levels, with values above a tenfold increase being the basis of the biopsy-free diagnostic approach suggested by present guidelines. This study showcased the largest screening project for CD carried out to date in school children (n=20,000) aimed at assessing the diagnostic accuracy of minimally invasive finger prick point-of-care tests (POCT) which, combined with conventional celiac serology and the aid of an artificial intelligence-based system, may eliminate t…
Special Issue on Signal Processing and Machine Learning for Biomedical Data
2021
This Special Issue is focused on advanced techniques in signal processing, analysis, modelling, and classification, applied to a variety of medical diagnostic problems. Biomedical data play a fundamental role in many fields of research and clinical practice. Very often the complexity of these data and their large volume makes it necessary to develop advanced analysis techniques and systems. Furthermore, the introduction of new techniques and methodologies for diagnostic purposes, especially in the field of medical imaging, requires new signal processing and machine learning methods. The recent progress in machine learning techniques, and in particular deep learning, revolutionized various f…
MAGIC-5: an Italian mammographic database of digitised images for research
2008
The implementation of a database of digitised mammograms is discussed. The digitised images were collected beginning in 1999 by a community of physicists in collaboration with radiologists in several Italian hospitals as a first step in developing and implementing a computer-aided detection (CAD) system. All 3,369 mammograms were collected from 967 patients and classified according to lesion type and morphology, breast tissue and pathology type. A dedicated graphical user interface was developed to visualise and process mammograms to support the medical diagnosis directly on a high-resolution screen. The database has been the starting point for developing other medical imaging applications,…
Window-Based Energy Selecting X-ray Imaging and Charge Sharing in Cadmium Zinc Telluride Linear Array Detectors for Contaminant Detection
2023
The spectroscopic and imaging performance of energy-resolved photon counting detectors, based on new sub-millimetre boron oxide encapsulated vertical Bridgman cadmium zinc telluride linear arrays, are presented in this work. The activities are in the framework of the AVATAR X project, planning the development of X-ray scanners for contaminant detection in food industry. The detectors, characterized by high spatial (250 µm) and energy (<3 keV) resolution, allow spectral X-ray imaging with interesting image quality improvements. The effects of charge sharing and energy-resolved techniques on contrast-to-noise ratio (CNR) enhancements are investigated. The benefits of a new energy-resolved …
A Microcalcification Detection System in Mammograms based on ANN Clustering
2018
Breast cancer is one of the leading causes to women mortality in the world. Clustered microcalcifications (MCs) in mammograms can be an important early sign of breast cancer, the detection is important to prevent and treat the disease. In this work, we present a novel method for the detection of MCs in mammograms which consists of regions of Interest (ROIs) segmentation, based on a spatial filter that allows the detection of small and large microcalcifications, clustering and classification of MCs by Artificial Neural Network. The system has been tested on a public dataset of digital images and compared with previous approaches. The results demonstrate that the proposed approach could achie…
A Wavelet approach to extract main features from indirect immunofluorescence images
2019
A number of previous studies have shown that IIF image analysis requires complex and sometimes heterogeneous and diversified methods. Robust solutions can be proposed but they need to orchestrate several methods from low-level analysis up to more complex neural networks or SVM for data classification. The contribution intends to highlight the versatility of Wavelet Transform (WT) and their use in various levels of analysis for the classification of IIF images in order to develop a system capable of performing: image enhancement, ROI segmentation and object classification. Therefore, WT was adopted in the de-noise section, segmentation and classification. This analysis allows frequencies cha…
Sedimentation of halloysite nanotubes from different deposits in aqueous media at variable ionic strengths
2019
Abstract Halloysite clay is a natural nanomaterial that is attracting a growing interest in colloidal science. The halloysite aqueous dispersion stability is a key aspect for the configuration of a purification protocol as well as to establish the durability of a formulation. A physico-chemical study demonstrated the role of ionic strength and nanotube characteristic sizes on the sedimentation behavior. We highlighted the importance of the electrostatic repulsions exercised between the particles in the settling process. A protocol for image analysis has been proposed to provide robust information from time resolved optical images on the suspensions. In conclusion, we managed to correlate mi…
A CAD system for nodule detection in low-dose lung CTs based on region growing and a new active contour model
2007
A computer-aided detection (CAD) system for the selection of lung nodules in computer tomography (CT) images is presented. The system is based on region growing (RG) algorithms and a new active contour model (ACM), implementing a local convex hull, able to draw the correct contour of the lung parenchyma and to include the pleural nodules. The CAD consists of three steps: (1) the lung parenchymal volume is segmented by means of a RG algorithm; the pleural nodules are included through the new ACM technique; (2) a RG algorithm is iteratively applied to the previously segmented volume in order to detect the candidate nodules; (3) a double-threshold cut and a neural network are applied to reduce…
Computer-aided diagnosis in digital mammography: comparison of two commercial systems
2014
Aim: Within this work, a comparative analysis of two commercial computer-aided detection or diagnosis (CAD) systems, CyclopusCAD® mammo (v. 6.0) produced by CyclopusCAD Ltd (Palermo, Italy) and SecondLook® (v. 6.1C) produced by iCAD Inc. (OH, USA) is performed by evaluating the results of both systems application on an unique set of mammographic digital images routinely acquired in a hospital structure. Materials & methods: The two CAD systems have been separately applied on a sample set of 126 mammographic digital cases, having been independently diagnosed by two senior radiologists. According to the human diagnosis, the cases in the sample reference set are divided into 61 negatives and 6…
Incomplete Charge Collection at Inter-Pixel Gap in Low- and High-Flux Cadmium Zinc Telluride Pixel Detectors.
2022
The success of cadmium zinc telluride (CZT) detectors in room-temperature spectroscopic X-ray imaging is now widely accepted. The most common CZT detectors are characterized by enhanced-charge transport properties of electrons, with mobility-lifetime products μeτe > 10−2 cm2/V and μhτh > 10−5 cm2/V. These materials, typically termed low-flux LF-CZT, are successfully used for thick electron-sensing detectors and in low-flux conditions. Recently, new CZT materials with hole mobility-lifetime product enhancements (μhτh > 10−4 cm2/V and μeτe > 10−3 cm2/V) have been fabricated for high-flux measurements (high-flux HF-CZT detectors).…
Fuzzy technique for microcalcifications clustering in digital mammograms
2012
Abstract Background Mammography has established itself as the most efficient technique for the identification of the pathological breast lesions. Among the various types of lesions, microcalcifications are the most difficult to identify since they are quite small (0.1-1.0 mm) and often poorly contrasted against an images background. Within this context, the Computer Aided Detection (CAD) systems could turn out to be very useful in breast cancer control. Methods In this paper we present a potentially powerful microcalcifications cluster enhancement method applicable to digital mammograms. The segmentation phase employs a form filter, obtained from LoG filter, to overcome the dependence from …
Comparative Study of Human and Automated Screening for Antinuclear Antibodies by Immunofluorescence on HEp-2 Cells
2015
Background : Several automated systems had been developed in order to reduce inter-observer variability in indirect immunofluorescence (IIF) interpretation. We aimed to evaluate the performance of a processing system in antinuclear antibodies (ANA) screening on HEp-2 cells. Patients and Methods : This study included 64 ANA-positive sera and 107 ANA-negative sera that underwent IIF on two commercial kits of HEp-2 cells (BioSystems® and Euroimmun®). IIF results were compared with a novel automated interpretation system, the “ Cyclopus CADImmuno®” (CAD). Results : All ANA-positive sera images were recognized as positive by CAD (sensitivity = 100%), while 17 (15.9%) of the ANA-negative sera ima…
Automatic detection of lung nodules in CT datasets based on stable 3D mass–spring models
2012
We propose a computer-aided detection (CAD) system which can detect small-sized (from 3 mm) pulmonary nodules in spiral CT scans. A pulmonary nodule is a small lesion in the lungs, round-shaped (parenchymal nodule) or worm-shaped (juxtapleural nodule). Both kinds of lesions have a radio-density greater than lung parenchyma, thus appearing white on the images. Lung nodules might indicate a lung cancer and their early stage detection arguably improves the patient survival rate. CT is considered to be the most accurate imaging modality for nodule detection. However, the large amount of data per examination makes the full analysis difficult, leading to omission of nodules by the radiologist. We…
Preliminary results of the project A.I.D.A. (Auto Immunity: Diagnosis Assisted by computer)
2014
In this paper, are presented the preliminary results of the A.I.D.A. (Auto Immunity: Diagnosis Assisted by computer) project which is developed in the frame of the cross-border cooperation Italy-Tunisia. According to the main objectives of this project, a database of interpreted Indirect ImmunoFluorescence (IIF) images on HEp 2 cells is being collected thanks to the contribution of Italian and Tunisian experts involved in routine diagnosis of autoimmune diseases. Through exchanging images and double reporting; a Gold Standard database, containing around 1000 double reported IIF images with different patterns including negative tests, has been settled. This Gold Standard database has been us…
Classifier trained on dissimilarity representation of medical pattern: A comparative study.
2005
In this paper we investigate the feasibility of some typical techniques of pattern recognition for the classification of medical examples. The learning of the classifiers is not made in the traditional features space but it can be made by constructing decision rules on dissimilarity (distance) representations. In such a recognition process a new object is described by its distances to (a subset of) the training samples. Purpose of this work is the development of an automatic classification system which could be useful for radiologists in the investigation of breast cancer. The software has been designed in the framework of the MAGIC-5 collaboration. In the automatic classification system th…
Fast Fourier Transform Filtering for Bilateral Mammography Comparison
2010
Bilateral Asymmetry is one of the breast abnormalities that may indicate a cancer in early stage. The computer methods for the bilateral subtraction developed up to now show the problem of large false positives number because the alignment defects. On the other hand the computer methods using FFT approach suffer of a low S/N ratio to distinguish massive lesions from background. In this paper a method (FFT-RF-BMC) is presented to enhanche the bilateral asymmetry using a FFT to detect massive lesions through a Recursive Filtering.
CT imaging applied to capillary water absorption in sicilian sedimentary rocks used in cultural heritages
2006
METODO AUTOMATICO PER LA RIVELAZIONE PRECOCE DI ZONE TUMORALI IN IMMAGINI BIOMEDICHE
2006
METHOD FOR PROCESSING BIOMEDICAL IMAGES
2007
The present invention relates to a method for highlight and to diagnose regions of interest in biomedical radiographic images, useful in the context of a CAD tool processing operating as recond reader during the normal clinical and screening routine, so to reducing the costs of management of the "double reading" procedure.
Dissimilarity Application for Medical Imaging Classification
2005
In the traditional way of learning from examples of objects the classifiers are built in a feature space. However, alternative ways can be found by constructing decision rules on dissimilarity (distance) representations. In such a recognition process a new object is described by its distances to (a subset of) die training samples. The use of the dissimilarities is especially of interest when features are difficult to obtain or when they have a little discriminative power. Purpose of this work is the development of an automatic classification system which could be useful for radiologists in the investigation of breast cancer. The software has been designed in the framework of the MAGIC-5 col…
Diagnosi assistita da computer (CAD) in mammografia. Confronto di accuratezza diagnostica di un nuovo algoritmo (Cyclopus®, Medicad) con due sistemi …
2009
Metodo di Template Matching per l'Analisi di Immagini
2012
La presente invenzione si riferisce ad un metodo di Template Matching per l’analisi di immagini da ImmunoFluorescenza Indiretta (IFI) per la rivelazione e classificazione automatica di pattern autoanticorpali. L’invenzione qui presentata generalizza il metodo del Template Matching operando innovativamente il mapping del contenuto visuale dell’immagine con particolari funzioni discrete qui denominate “mappatori”; inoltre, utilizzando le informazioni provenienti dalla sovrapposizione dei vari mappatori con un metodo di confronto funzionale, realizza una funzione di correlazione originale. La metodologia descritta nel seguito presenta una flessibilità tale da renderla applicabile a qualsiasi p…