First stages of microsporere programming to embryogenesis through anther culture in Prunus armeniaca L.

6 páginas, 3 figuras, 2 tablas -- PAGS nros. 152-157

Coltura in vitro di microspore isolate per il miglioramento genetico di Olea europaea L.

Gli aploidi (piante con un numero gametofitico di cromosomi nello sporofito: n invece di 2n) ed i doppio-aploidi (aploidi che hanno raddoppiato il numero di cromosomi spontaneamente o in maniera indotta) trovano numerose applicazioni nel miglioramento genetico vegetale, ed in particolare, nella mutagenesi, nella selezione in vitro, nella trasformazione, nell’utilizzo della variabilità gametoclonale ed anche nel sequenziamento del genoma e negli studi di genomica funzionale. Pertanto, essi costituiscono uno strumento biotecnologico particolarmente interessante nel miglioramento genetico delle piante. Ai fini dell’ottenimento dell’omozigosi nell’olivo, come in tutte le piante arboree, caratte…

FIRST STAGES OF MICROSPORE REPROGRAMMING TO EMBRYOGENESIS THROUGH ISOLATED MICROSPORE CULTURE IN LOQUAT

The current experiments were undertaken to develop a method for regenerating doubled haploid (DH) plants of Eriobotrya japonica (Thunb.), Rosaceae, subfamily Maloideae through isolated microspore culture of nine loquat cultivars. Protocols were developed for isolation and culture of loquat microspores. Isolated microspores in vitro cultured started dividing and developing multicellular, globular and irregular structures. After 4 weeks of culture, the responding microspores produced yellowish/white callus. The embryogenic response of microspores was highly dependent on the cultivar and on the medium composition.

Induction of pollen embryogenesis in seven cultivars of Prunus armeniaca l.

6 páginas -- PAGS nros. 273-278

Caratterizzazione chimico-fisica e sensoriale di frutti di mango (Mangifera indica L.) coltivati in Sicilia .

OCCURRENCE OF SPONTANEOUS TRIPLOIDS FROM SMALL SEEDS OF 'HERNANDINA' CLEMENTINE

In Vitro Plant Regeneration from Epicotyls Segments of Carrizo Citrange.

Ploidy levels in Citrus clementine affects leaf morphology, stomatal density and water content

The objective of the present study was to understand the relationship among leaf morphology, stomatal characteristics and water relations in triploids generated through anther culture and their counterpart diploid plant of C. clementina. Triploid plants possessed small and narrow leaves as compared to diploid plant as evident by less leaf length, leaf width and leaf area. By contrast, the leaf index was observed to be more in triploids than haploid ones. Flow cytometric analysis re-confirmed the ploidy levels of heterozygous plant Hd as diploid and the ploidy of Th1, Th2, Th3 and Th4 plants as triploids. A positive relation was found between ploidy level and stomatal guard cell length and w…

Caratteristiche qualitative di frutti di Lici (Litchi Chinensis) coltivati nella Sicilia Nord-Orientale

Valutazione della variabilità genetica di alcune cultivar di nespolo del Giappone mediante SSRs e AFLP..

Gametic embryogenesis in Mediterranean fruit crops

Contenuto minerale delle foglie e qualità dei frutti di agrumi per la trasformazione

In the present study, we analyzed leaf mineral contents fruit quality traits of orange, lemon and tangerine varieties grown in Sicily. The purpose of this research was to individuate chemical or physical fruit quality parameters useful to establish the best possible use of the crop, fresh market or processing. Moreover, leaf mineral analyses were conducted in order to characterize nutritional levels of citrus varieties grown in Sicily. In a second study, we evaluated fruit quality of orange trees cv Valencia in response to deficit irrigation.

First stages of microspore reprogramming to embryogenesis through anther culture in Prunus armeniaca L