Different Within-Host Viral Evolution Dynamics in Severely Immunosuppressed Cases with Persistent SARS-CoV-2

12 páginas, 2 figuras, 1 tabla.

Characterization of 10 trinucleotide microsatellite loci in the Critically Endangered Pyrenean yam Borderea chouardii (Dioscoreaceae)

The low levels of allozymic variability found in the Critically Endangered Borderea chouardii prompted us to develop microsatellite markers to assess the genetic variability and population structure for the adequate conservation management of this species. A (CTT) n enriched partial genomic library was constructed. Ten polymorphic microsatellite loci were isolated from it, rendering 51 alleles in 47 individuals analysed. The allelic pattern observed for all of the loci with more than two alleles suggests that B. chouardii is tetraploid.

On the verge of extinction: genetics of the critically endangered Iberian plant species, Borderea chouardii (Dioscoreaceae) and implications for conservation management

Borderea chouardii is a relictual and dioecious, strictly sexually reproducing, long-living geophyte of the Dioscoreaceae family. Previous biological and demographic studies have indicated the existence of a uniformly distributed panmictic population of this taxon at the southernmost Spanish pre-Pyrenean mountain ranges where it occurs in rather inaccessible crevices of a single limestone cliff. However, individuals of B. chouardii are spatially subdivided into two subpopulations located, respectively, on the upper and lower parts of the cliff, and vertically separated 150 m. Because of its extreme rarity, B. chouardii was the first Iberian taxon to have a specific conservation plan and has…

Late Cretaceous-Early Eocene origin of yams (Dioscorea, Dioscoreaceae) in the Laurasian Palaearctic and their subsequent Oligocene-Miocene diversification

Aim: Dioscorea (Dioscoreaceae) is a predominantly pantropical genus (< 600 species) that includes the third most important tropical tuber crop and species of pharmacological value. Fossil records from both the Northern and Southern Hemispheres were used to test hypotheses about the origin of the genus Dioscorea, and to examine potential macroevolutionary processes that led to its current distribution. Location: Pantropical distribution. Methods: Divergence times were estimated using the most comprehensive phylogeny of the group published to date based on plastid sequences and fossil calibrations, applying a relaxed-clock model approach. Ancestral areas and range shifts were reconstructed us…

Nunatak survival vs. tabula rasa in the Central Pyrenees: a study on the endemic plant species Borderea pyrenaica (Dioscoreaceae)

14 páginas, 4 figuras, 2 tablas.

Past climate changes facilitated homoploid speciation in three mountain spiny fescues (Festuca, Poaceae)

Apart from the overwhelming cases of allopolyploidization, the impact of speciation through homoploid hybridization is becoming more relevant than previously thought. Much less is known, however, about the impact of climate changes as a driven factor of speciation. To investigate these issues, we selected Festuca picoeuropeana, an hypothetical natural hybrid between the diploid species F. eskia and F. gautieri that occurs in two different mountain ranges (Cantabrian Mountains and Pyrenees) separated by more than 400 km. To unravel the outcomes of this mode of speciation and the impact of climate during speciation we used a multidisciplinary approach combining genome size and chromosome coun…

Overlapping of Independent SARS-CoV-2 Nosocomial Transmissions in a Complex Outbreak

8 páginas, 2 figuras, 1 tabla.

A nuclear Xdh phylogenetic analysis of yams (Dioscorea: Dioscoreaceae) congruent with plastid trees reveals a new Neotropical lineage

No evidence of adverse cardiac remodeling in former elite endurance athletes

Background: The impact of high exercise loads on a previously healthy heart remains controversial. We examined the consequences of decades of strenuous endurance exercise at the highest competition level on heart dimensions and volumes as well as on serum biomarkers of cardiac fibrosis/remodeling. Methods and results: We compared echocardiographic measurements and serum biomarkers of cardiac fibrosis/remodeling [troponin I, galectin-3, matrix metallopeptidase-2 and -9, N-terminal pro-brain natriuretic peptide, carboxy-terminal propeptide of type I procollagen, and soluble suppressor of tumorigenicity-2 (sST-2)/interleukin(IL)-1R4] in 53 male athletes [11 former professional ('elite') and 42…

Proper assignation of reactivation in a COVID-19 recurrence initially interpreted as a reinfection

A 77-year-old-male (Case R) who had had a previous diagnosis of mild COVID-19 episode, was hospitalized 35 days later. On Day 23 post-admission, he developed a second COVID-19 episode, now severe, and finally died. Initially, Case R COVID-19 recurrence was interpreted as a reinfection due to the exposure to a SARS-CoV-2 RT-PCR-positive room-mate. However, whole-genome-sequencing indicated that case R recurrence corresponded to a reactivation of the strain involved in his first episode. Case R reactivation had major consequences, leading to a more severe episode, and causing a subsequent transmission to another two hospitalized patients, one of them with fatal outcome.

Genotyping of a nosocomial outbreak of pandemic influenza A/H1N1 2009

Background: Epidemiological surveys have revealed outbreaks of pandemic influenza A (H1N1) 2009 in several different contexts. Molecular characterization of the influenza virus could help to provide a more accurate description of these outbreaks. Objective: To genotype pandemic influenza A (H1N1) 2009 isolates from an epidemiologically defined nosocomial outbreak. Study design: We sequenced the neuraminidase (NA) and hemagglutinin (HA) influenza A (H1N1) 2009 genes from ten HIV-positive patients involved in an epidemiologically defined outbreak in the Clinical Microbiology and Infectious Diseases (CMID) Department. Sequences were aligned to search for specific genetic features of the involv…

Complete analysis of the epidemiological scenario around a SARS-CoV-2 reinfection: previous infection events and subsequent transmission

9 páginas, 3 figuras. The data that support the findings of this study (Fastq files) are publicly available. Fastq files above the GISAID thresholds were deposited at GISAID (hCoV-19/Spain/MD-IBV-99007733/2020, hCoV-19/Spain/MD-IBV-99007151/2020, hCoV-19/Spain/MD-IBV-99007734/2020, and hCoV-19/Spain/MDIBV-99007170/2020). All sequences were also deposited at the ENA (European Nucleotide Archive; https:// www.ebi.ac.uk/ena/browser/home) (ERR5698024, ERR5697187, ERR6459974, ERR5698025, and ERR5697254).

Strenuous endurance exercise and right ventricular systolic function: no evidence of long-term sequelae.

a Faculty of Medicine, Department of Physiology, University of Valencia, Valencia, Spain b Fundacion de Investigacion del Hospital Clinico Universitario Valencia (FIHCUV-INCLIVA), Valencia, Spain c Faculty of Health and Sport Science, University of Zaragoza, Huesca, Spain d Hospital Universitario 12 de Octubre, Research Institute (i + 12), Madrid, Spain e HospitalUniversitario Miguel Servet, Zaragoza, Spain f Cardiology Service, Hospital Universitario Miguel Servet, Zaragoza, Spain g European University of Madrid, Madrid, Spain h Faculty of Medicine, Physical Education and Sports Section, University of Zaragoza, Zaragoza, Spain