0000000001001359

AUTHOR

H. Arvilommi

showing 2 related works from this author

Leukocyte chemotactic activity in cultures of unstimulated human lymphocytes.

2009

We have shown earlier that unstimulated human lymphocytes in in vitro cultures produce migration inhibitory factor into the supernatant. The evidence of spontaneous lymphokine synthesis is strengthened further by this study, which demonstrates leukocyte chemotactic activity in these culture supernatants. The factor has a molecular weight of more than 5000 daltons, it resisted heating for 15 min at 100 degrees C, and showed maximum activity at dilution 1:4-1:8 of the supernatants.

medicine.medical_specialtyLymphokinesHot TemperatureChemistryLymphokineChemotaxisGeneral MedicineMolecular biologyIn vitroMonocytesMigration Inhibitory FactorMolecular WeightChemotaxis LeukocyteEndocrinologyInternal medicinemedicineHumansCell culture supernatantLymphocytesCells CulturedActa pathologica et microbiologica Scandinavica. Section C, Immunology
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A Simple Technique for Measuring Leucocyte Chemotaxis in Reversible Boyden Chambers

1977

A technique for assay of leucocyte chemotaxis in Boyden chambers is described. A set of up to 12 closed chambers with 2 compartments is formed from commercially available migration plates. The set can be turned during the incubation and thus the detachment of cells from the filter, a major source of variation in classical type of chambers, can be prevented. The technique is simple and so inexpensive that it allows the use of the material as disposable if preferred.

ChromatographyChemistryLeucocyte chemotaxisAnalytical chemistryfood and beveragesGeneral MedicineClassical typeZeitschrift für Immunitätsforschung: Immunobiology
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