6533b856fe1ef96bd12b30e1

RESEARCH PRODUCT

Leukocyte chemotactic activity in cultures of unstimulated human lymphocytes.

H. ArvilommiH. ArvilommiAino LaatikainenAino LaatikainenLiisa RäsänenLiisa Räsänen

subject

medicine.medical_specialtyLymphokinesHot TemperatureChemistryLymphokineChemotaxisGeneral MedicineMolecular biologyIn vitroMonocytesMigration Inhibitory FactorMolecular WeightChemotaxis LeukocyteEndocrinologyInternal medicinemedicineHumansCell culture supernatantLymphocytesCells Cultured

description

We have shown earlier that unstimulated human lymphocytes in in vitro cultures produce migration inhibitory factor into the supernatant. The evidence of spontaneous lymphokine synthesis is strengthened further by this study, which demonstrates leukocyte chemotactic activity in these culture supernatants. The factor has a molecular weight of more than 5000 daltons, it resisted heating for 15 min at 100 degrees C, and showed maximum activity at dilution 1:4-1:8 of the supernatants.

10.1111/j.1699-0463.1978.tb02559.xhttps://pubmed.ncbi.nlm.nih.gov/696331