Sperm kinematics and morphometric subpopulations analysis with CASA systems: a review

Sperm kinematics and morphometric subpopulations analysis with CASA systems: a review. The subjective evaluation of seminal quality has given way to the use of objective assessment techniques by CASA technology (computer-assisted semen analysis). The application of principal components (PC) and clustering methods to reveal subpopulations of spermatozoa is a powerful tool to evaluate raw semen and processed cell suspensions, but not many researchers are aware of the technique. PC analysis is a multivariate statistical method that reduces the number of variables used in subsequent calculations used to describe the data. By integrating the original variables according to their coherence in a d…