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RESEARCH PRODUCT

Sperm kinematics and morphometric subpopulations analysis with CASA systems: a review

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Sperm kinematics and morphometric subpopulations analysis with CASA systems: a review. The subjective evaluation of seminal quality has given way to the use of objective assessment techniques by CASA technology (computer-assisted semen analysis). The application of principal components (PC) and clustering methods to reveal subpopulations of spermatozoa is a powerful tool to evaluate raw semen and processed cell suspensions, but not many researchers are aware of the technique. PC analysis is a multivariate statistical method that reduces the number of variables used in subsequent calculations used to describe the data. By integrating the original variables according to their coherence in a database into a new complex mathematical variable, clearly defined homogenous subpopulations of spermatozoa can be defined. Kinematic, morphometric, morphological or DNA integrity tests may apply to characterize it and understand the reproductive biology of the spermatozoon. In recent years there has been a substantive change in the conceptual paradigm regarding what an ejaculate is. It should be said that until recently it was considered that the representative population (billions) of sperm was made up of "equivalent" cells with a common goal: to be the one that could finally fertilize the oocyte. The verification that the set of spermatozoa is grouped in distinct subpopulations, according to their kinematic and morphometric characteristics, opens the way towards a more cooperative vision. Besides, we have seen that the subpopulation distribution is different among males, which seems to indicate different strategies that can be understood within another paradigm, that of sperm competition between different ejaculates. The existence of heterogeneous subpopulations of spermatozoa in the ejaculate that show kinematics and morphometric patterns is widely known but the biological meaning of these different sperm subpopulations is still not clear. Although the role of the different subpopulations remains unknown, the work should continue in that direction. Análisis de subpoblaciones cinéticas y morfométricas de espermatozoides con sistemas CASA: una revisión. La valoración subjetiva de la calidad seminal ha dado paso al uso de las técnicas objetivas de valoración por medio de la tecnología CASA (computer-assisted semen analysis). Se pueden aplicar una serie de pruebas cinéticas, morfométricas, morfológicas o de integridad del DNA para caracterizar y entender la biología reproductiva del espermatozoide. En los últimos años se ha dado un cambio sustantivo de paradigma conceptual respecto de qué es un eyaculado. Cabe decir que hasta tiempos recientes se consideró que la representativa población (miles de millones) de espermatozoides estaba formado por células "equivalentes" con un objetivo común: ser el que finalmente pudiese fecundar el ovocito. La comprobación de que el conjunto de espermatozoides se agrupa en subpoblaciones bien definidas, de acuerdo con sus características cinéticas y/o morfométricas abre el camino hacia una visión más cooperativa. Además, se ha visto que la distribución subpoblacional es diferente entre individuos, lo que parece indicar diferentes estrategias que se pueden entender dentro de otro paradigma, el de la competencia espermática entre diferentes eyaculados. A pesar de que aún no se conoce el papel de las diferentes subpoblaciones o, se deben continuar los trabajos en esa dirección.