Author: Kristiāna Kovtuna

0000000001039006

AUTHOR

Kristiāna Kovtuna

showing 2 related works from this author

Rauga Kluyveromyces marxianus acetāta tolerance

2017

Maģistra darba mērķis bija novērtēt rauga Kluyveromyces marxianus iespējamos šūnu adaptācijas mehānismus pret acetāta izraisīto stresu. Fermentācijās ar laktozi saturošu substrātu un pievienotu 40 mM acetātu šūnu kultūrai tika novērota paildzināta lag fāze, kuras laikā palielinājās šūnu populācijas rezistence pret acetātu. Pētījumos tika novērota rauga Kluyveromyces marxianus DSM 5422 šūnu populācijas heterogenitāte, kas liecina, ka daļa no šūnām sākotnēji ir tolerantāka pret organisko skābju izraisīto stresu. Kultivējot raugu K. marxianus laktozi saturošā barotnē ar pievienotu acetātu, tika novērota iekššūnu vides pH palielināšanās. Iespējams, raugam K. marxianus vājo skābju izraisīta stre…

etiķskābelaktozeKluyveromyces marxianusvājo organisko skābju stressβ-galaktozidāzeBioloģija

researchProduct

Rauga Kluyveromyces marxianus biomasas producēšana laktozi saturošos substrātos

2015

Bakalaura darba mērķis bija izvērtēt aerācijas, temperatūras, rauga ekstrakta, melases, amonija sulfāta un urīnvielas ietekmi uz biomasas producēšanu un tās sastāvu laktozes saturošās barotnēs. Fermentācijām tika izvēlēts rauga Kluyveromyces marxianus DSM 5422 celms, kas uzrādīja labāku biomasas iznākumu, salīdzinot ar rauga Kluyveromyces lactis DSM 4394, DSM 70800 un DSM 70799 celmiem. Aerācijas diapazonā 0,2 – 2,0 L·L-1·min-1 optimāls biomasas iznākums tika novērots 1,0 L·L-1·min-1 aerācijas režīmā 35oC temperatūrā. Tika pētīta dažādu piedevu (rauga ekstrakta, melases, amonija sulfāta, urīnvielas) ietekme uz biomasas veidošanos. Rauga ekstrakta un melases pievienošana fermentācijas barotn…

laktozefermentācijabiomasasiera sūkalasKluyveromyces marxianusBioloģija

researchProduct

PEPhysical Sciences and Engineering
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LSLife Sciences
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