Author: Laurent Brechenmacher

0000000001068527

AUTHOR

Laurent Brechenmacher

showing 8 related works from this author

Construction and validation of cDNA-based Mt6k-RIT macro- and microarrays to explore root endosymbioses in the model legume Medicago truncatula

2004

To construct macro- and microarray tools suitable for expression profiling in root endosymbioses of the model legume Medicago truncatula, we PCR-amplified a total of 6048 cDNA probes representing genes expressed in uninfected roots, mycorrhizal roots and young root nodules [Nucleic Acids Res. 30 (2002) 5579]. Including additional probes for either tissue-specific or constitutively expressed control genes, 5651 successfully amplified gene-specific probes were used to grid macro- and to spot microarrays designated Mt6k-RIT (M. truncatula 6k root interaction transcriptome). Subsequent to a technical validation of microarray printing, we performed two pilot expression profiling experiments usin…

0106 biological sciencesRoot nodule[SDV]Life Sciences [q-bio]Plant Roots01 natural sciencesApplied Microbiology and BiotechnologyTranscriptomeADNCGene Expression Regulation PlantGene Expression Regulation FungalMycorrhizaeMedicagoPCR-basedComputingMilieux_MISCELLANEOUSOligonucleotide Array Sequence AnalysisPlant ProteinsExpressed Sequence Tags2. Zero hunger0303 health sciencesnodulin genesroot nodule symbiosisarbuscular mycorrhizafood and beveragesEquipment DesignGeneral MedicineMedicago truncatulaArbuscular mycorrhiza[SDV] Life Sciences [q-bio]expression profilingDNA microarrayBiotechnologyBioengineeringComputational biologyBiologySensitivity and Specificity03 medical and health sciencesComplementary DNABotanySymbiosisLeghemoglobin030304 developmental biologyGene Expression ProfilingfungiReproducibility of Resultsbiology.organism_classificationEquipment Failure AnalysisGene expression profilingphosphate transportercDNA array010606 plant biology & botany

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Plant genes involved in arbuscular mycorrhiza formation and functioning

2002

Knowledge about that part of the plant genome involved in the establishment and functioning of the arbuscular mycorrhizal (AM) symbiosis is important for the basic understanding of this symbiosis. It is also essential for a ‘genes to the field’ approach based on the identification and exploitation of genes that could be central to developing sustainable plant production systems in the future.

0106 biological sciences0303 health sciencesbiologybusiness.industry[SDV]Life Sciences [q-bio]biology.organism_classification01 natural sciencesGenomeBiotechnologyArbuscular mycorrhiza[SDV] Life Sciences [q-bio]03 medical and health sciencesSymbiosisBotanyREPONSE DE LA PLANTEIdentification (biology)Arbuscular mycorrhizalbusinessPlant genesGeneFunctional genomicsComputingMilieux_MISCELLANEOUS030304 developmental biology010606 plant biology & botany

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Functional genomics of arbuscular mycorrhiza : decoding the symbiotic cell programme

2004

More extensive insight into plant genes involved in the symbiotic programme of arbuscular mycorrhiza is presently being achieved by global approaches that aim to discover novel genes or subsets of genes that are essential to cell programmes in the different steps of plantfungal interactions. The strategy of functional genomics based on large-scale differential RNA expression analyses (differential-display reverse transcriptase - PCR), electronic Northerns, suppressive subtractive hybridization, DNA chips) is presented, with a focus on arbuscular mycorrhiza in Pisum sativum and Medicago truncatula. The most recent knowledge about gene networks that are modulated in roots during arbuscular …