0000000001107818

AUTHOR

Beatriz Galán

showing 4 related works from this author

Identification and expression of the 11β‐steroid hydroxylase from Cochliobolus lunatus in Corynebacterium glutamicum

2019

Hydroxylation of steroids has acquired special relevance for the pharmaceutical industries. Particularly, the 11β-hydroxylation of steroids is a reaction of biotechnological importance currently carried out at industrial scale by the fungus Cochliobolus lunatus. In this work, we have identified the genes encoding the cytochrome CYP103168 and the reductase CPR64795 of C. lunatus responsible for the 11β-hydroxylase activity in this fungus, which is the key step for the preparative synthesis of cortisol in industry. A recombinant Corynebacterium glutamicum strain harbouring a plasmid expressing both genes forming a synthetic bacterial operon was able to 11β-hydroxylate several steroids as subs…

Operonlcsh:BiotechnologyGenetic VectorsGene ExpressionBioengineeringReductaseHydroxylationApplied Microbiology and BiotechnologyBiochemistryCorynebacterium glutamicumHydroxylation03 medical and health scienceschemistry.chemical_compoundPlasmidBiotransformationAscomycotalcsh:TP248.13-248.65Cloning MolecularResearch ArticlesBiotransformation030304 developmental biology0303 health sciencesbiology030306 microbiologyCochliobolus lunatusbiology.organism_classificationRecombinant ProteinsCorynebacterium glutamicumchemistryBiochemistrySteroid hydroxylaseSteroid 11-beta-HydroxylasebacteriaSteroidsBiotechnologyResearch ArticlePlasmidsMicrobial Biotechnology
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Microalgae, old sustainable food and fashion nutraceuticals.

2017

8 p.-1 fig.

0106 biological sciences0301 basic medicineOpinionbusiness.industryNatural resource economicsIndustrial scaleBioengineeringHealth benefits01 natural sciencesApplied Microbiology and BiotechnologyBiochemistryBiotechnology03 medical and health sciences030104 developmental biologyNutraceutical010608 biotechnologySustainable agricultureDietary SupplementsMicroalgaeAnimalsHumansBusinessSDG 2: End hunger achieve food security and improved nutrition and promote sustainable agricultureNutritive ValueBiotechnologyMicrobial biotechnology
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High-Quality Whole-Genome Sequence of an Estradiol-Degrading Strain, Novosphingobium tardaugens NBRC 16725

2019

In this work we report the complete sequence and assembly of the estradiol-degrading bacterium Novosphingobium tardaugens NBRC 16725 genome into a single contig using the Pacific Biosciences RS II system.

Sewage-treatment plant010501 environmental sciencesBiologymedicine.disease_cause01 natural sciencesGenomeDegradation03 medical and health sciencesComplete sequenceImmunology and Microbiology (miscellaneous)GeneticsmedicineMolecular Biology030304 developmental biology0105 earth and related environmental sciencesGeneticsWhole genome sequencing0303 health sciencesBacteriaStrain (chemistry)ContigGenome Sequencesfood and beveragesbiology.organism_classificationNovosphingobium tardaugensActivated-sludgePacific biosciencesBacteriaMicrobiology Resource Announcements
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Genome Sequence of the Methanotrophic Poly-β-Hydroxybutyrate Producer Methylocystis parvus OBBP

2012

-- PAGS 2 5709-5710

DNA Bacterialfood.ingredientOperonMethane monooxygenasePolyestersMolecular Sequence DataMethylocystisHydroxybutyratesmonooxigenasaMicrobiologyfoodmethylotrophOperonBotanyMolecular BiologyGeneGeneticsWhole genome sequencingbiologySequence Analysis DNAbiology.organism_classificationGenome AnnouncementsType speciesMethylocystisOxygenasesbiology.proteinMethylotrophMethylocystis parvusMethaneMethylocystaceaeGenome BacterialMetabolic Networks and PathwaysJournal of Bacteriology
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