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RESEARCH PRODUCT
Identification and expression of the 11β‐steroid hydroxylase from Cochliobolus lunatus in Corynebacterium glutamicum
José Luis GarcíaJosé Luis GarcíaJosé M. LuengoFrancisco J. MedranoCarmen Felpeto-santeroBeatriz GalánCarlos Del CerroJosé M. Fernández-cañónsubject
Operonlcsh:BiotechnologyGenetic VectorsGene ExpressionBioengineeringReductaseHydroxylationApplied Microbiology and BiotechnologyBiochemistryCorynebacterium glutamicumHydroxylation03 medical and health scienceschemistry.chemical_compoundPlasmidBiotransformationAscomycotalcsh:TP248.13-248.65Cloning MolecularResearch ArticlesBiotransformation030304 developmental biology0303 health sciencesbiology030306 microbiologyCochliobolus lunatusbiology.organism_classificationRecombinant ProteinsCorynebacterium glutamicumchemistryBiochemistrySteroid hydroxylaseSteroid 11-beta-HydroxylasebacteriaSteroidsBiotechnologyResearch ArticlePlasmidsdescription
Hydroxylation of steroids has acquired special relevance for the pharmaceutical industries. Particularly, the 11β-hydroxylation of steroids is a reaction of biotechnological importance currently carried out at industrial scale by the fungus Cochliobolus lunatus. In this work, we have identified the genes encoding the cytochrome CYP103168 and the reductase CPR64795 of C. lunatus responsible for the 11β-hydroxylase activity in this fungus, which is the key step for the preparative synthesis of cortisol in industry. A recombinant Corynebacterium glutamicum strain harbouring a plasmid expressing both genes forming a synthetic bacterial operon was able to 11β-hydroxylate several steroids as substrates. This is a new example to show that the industrial strain C. glutamicum can be used as a suitable chassis to perform steroid biotransformation expressing eukaryotic cytochromes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-06-01 | Microbial Biotechnology |