Advances in detection of fastidious bacteria: From microscopic observation to molecular biosensors

Abstract Identification of pathogens and diagnosis of infections are important health challenges, especially in the case of fastidious bacteria which are those difficult-to-grow. A fastidious organism is any organism that has a complex nutritional requirement. Additionally, a fastidious microorganism will only grow when specific nutrients are included in the culture medium. These bacteria can cause serious diseases whose detection and monitoring is critical in many cases. The oldest detection methods are based on simple microscopy observation and staining, after culture on selective growth media, but often do not provide a clear answer. Some new molecular approaches, such as DNA-based seque…