6533b85ffe1ef96bd12c196b
RESEARCH PRODUCT
Advances in detection of fastidious bacteria: From microscopic observation to molecular biosensors
Mohammad Ahangarzadeh RezaeeJafar MosaferAhad MokhtarzadehBehzad BaradaranMiguel De La GuardiaMohammad HasanzadehMichael R HamblinMichael R. HamblinAhmad MobedMohammad Agazadehsubject
Fastidious organismbiology010401 analytical chemistrybiology.organism_classification01 natural sciences0104 chemical sciencesAnalytical ChemistryMicroscopic observationMicrobiologyIdentification (biology)Simple MicroscopySpectroscopyOrganismBacteriadescription
Abstract Identification of pathogens and diagnosis of infections are important health challenges, especially in the case of fastidious bacteria which are those difficult-to-grow. A fastidious organism is any organism that has a complex nutritional requirement. Additionally, a fastidious microorganism will only grow when specific nutrients are included in the culture medium. These bacteria can cause serious diseases whose detection and monitoring is critical in many cases. The oldest detection methods are based on simple microscopy observation and staining, after culture on selective growth media, but often do not provide a clear answer. Some new molecular approaches, such as DNA-based sequencing and antibody-antigen binding, are particularly promising in the case of fastidious bacteria. The objective of this review is to summarize the advantages of the use of biosensors as a modern diagnostic tool in microbiology for detecting fastidious bacteria.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-04-01 | TrAC Trends in Analytical Chemistry |