Arteriosclerosi ed artrite reumatoide: ruolo della terapia biologica

The recent literature about micronuclei. Consideration and reliefs.

Expression of Cyclooxygenase-1 and Cyclooxygenase-2 in normal and pathological human oral mucosa

Abstract: Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins (PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cell homeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is strongly expressed in inflammation. The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosal reactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positive outcome of oral cancer; therefore it would be useful to identify molecular markers whose expr…

Disfunzione diastolica in pazienti affetti da artrite reumatoide:ruolo della terapia con anti-TNF

Artropatia sclerodermica ed artrite reumatoide: non evidenza di overlap

Atrial Natriuretic Peptide presence in parotid gland of human fetus at 13th week of development and in adult man

Our immunohistochemical study shows that atrial natriuretic peptide (ANP) is present in both human fetal and adult parotid gland. In the fetuses ANP is localized in cell clusters, while in adult parotid gland, the ANP is seen only in the wall of intra- and extralobular ducts. The results suggest that ANP might play a role in the differentiation of the parotid gland similarly as in brain and that during fetal growth ANP may play a role in the regulating the secretion of primitive salivary fluid, even if acini are still not developed. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 55-58)

La determinazione degli anticorpi anti-peptide ciclico citrullinato è utile nel discriminare pazienti con artrite reumatoide da quelli con poliartrite associata a tiroidite cronica autoimmune

Presence of Oxytocin, Vasopressin and Atrial Natriuretic Peptide and Their Modification in Rat Hypothalamic Paraventricular Nucleus During Resistance Training

Summary Many studies have demonstrated the physiological effects of oxytocin (OT), atrial natriuretic peptide (ANP) and vasopressin (VP) in the homoeostasis of body fluids during physical exercise. However, a little information is available about the related immunohistochemical changes in hypothalamic magnocellular neurosecretory system during and after the training. The aim of the present work was to study the immunohistochemical changes in OT, ANP and VP levels in the hypothalamic paraventricular nucleus during and after resistance exercise protocol. Three groups of Wistar rats were trained by a rung ladder protocol for 15, 30 and 45 days, respectively; a fourth group was left to rest for…

Cigarette smoke increases BLT2 receptor functions in bronchial epithelial cells: in vitro and ex vivo evidence

Summary Leukotriene B4 (LTB4) is a neutrophil chemotactic molecule with important involvement in the inflammatory responses of chronic obstructive pulmonary disease (COPD). Airway epithelium is emerging as a regulator of innate immune responses to a variety of insults including cigarette smoke, the major risk factor for COPD. In this study we have explored whether cigarette smoke extracts (CSE) or soluble mediators present in distal lung fluid samples (mini-bronchoalveolar lavages) from smokers alter the expression of the LTB4 receptor 2 (BLT2) and peroxisome proliferator-activated receptor-α (PPAR-α) in bronchial epithelial cells. We also evaluated the effects of CSE on the expression of i…

Sicurezza dell'associazione Etanercept/Lamivudina in un paziente con artrite reumatoide ed infezione cronica da HBV

Atrian natriuretic peptide (ANP)-Presence in human inferior turbinate after botulism toxin type A treatment

Studies demomstrated the ANP-presence in the mucosa of human turbinate, the study aimed to investigate about the ANP-presence of human turbinates of patients after treatment with the Botulinum Toxyn-A(BTX-A) since no data are reported. Results show that in BTX-A treated patients ANP is present in the ciliated and basal cells of the epithelium of tonaca mucosa and some glands;therefore ANP-presence is more intense than in mucosa of untreated patients.

Inhalatory anaesthesia Vs. TIVA in oral surgery and laryngectomy with lateral clearing: our experience

ANP and insulin presence in human foetal pancreas

Aim of this work is to investigate the presence of Atrial natriuretic peptide (ANP) and insulin in human foetal pancreas from not diabetic and diabetic mothers. The data literature evidenced that relationship between serum ANP and insulin. This work carried out on the sections immunostained for ANP and nsulin showed positivity for ANP and insulin in beta-cells of pancreas from diabetic mothers, while positivity for insulin in beta-cells of foetuses from not diabetic mothers. Results indicate that the insulin presence stimulates the ANP synthesis in pancreas of human foetuses from diabetic mothers. Aim of this work is to investigate the presence of Atrial natriuretic peptide (ANP) and insuli…

Expression of metalloproteinases (MMP-2, MMP-9) and cycloxygenases (COX-1, COX-2) in salivary glands tumors.

The somatotopy of the spinal cord: a comprehensive descrption.

For the benefit of medical students, in this paper we examine the somatotopic organization of the spinal cord, which shows a similar organization in mammalians. The dorsal horn is comprised of sensory nuclei with deutoneurons; the lateral horn contains preganglionic visceral motoneurons; the ventral horn is subdivided into a medial column innervating the paravertebral muscles, a central column, C3-C6, innervating the diaphragm, and a lateral column innervating the limb muscles. In the lateral column of the ventral horn, the dorsal motoneuron groups innervate the intrinsc arm muscles; the dorsal posterior motoneuron groups innervate hand muscles; the lateroventral motoneuron groups innervate…

Efficacia e sicurezza del trattamento con inibitori del TNF in pazienti affetti da spondilite anchilosante: uno studio osservazionale a 2 anni.

Atrial Natriuretic Peptide (ANP)-Presence in Human Inferior Turbinate after Treatment Botulinum Toxin Type A Treatment

La letteratura recente sui micronuclei. Considerazioni e rilievi The recent literature about micronuclei. Considerations and reliefs

Expression of MMP-2 and MMP-9 in odontogenic myxoma in a child: report of a clinical case

Odontogenic myxoma (OM) is a benign, locally invasive, non-metastasizing neoplasm of the jaw bones. Despite the benign nature of these lesions, there is a high rate of recurrence and the current recommended therapy, depending on the size and behaviour of the lesion, can vary from curettage with peripheral ostectomy, segmental resection up to radical resections for more aggressive lesions. OM is a rare tumour which occurs predominantly in the third decade of life and it is rare in children. Matrix metalloproteinases (MMPs) are a family of extracellular endopeptidases responsible for the degradation and remodelling of extracellular matrix, they are known to be involved in the progression and …

Aterosclerosi e artrite reumatoide: ruolo della twerapia biologica

La determinazione degli anticorpi antipeptide, C citrullinato è utile nel discriminare pazienti con artrite reumatoide da quelli con poliartrite associata a tiroidite cronica autoimmune.

Atrial natriuretic peptide (ANP) and oxytocin-expression in the adult rat and mouse cerebellum.

Abstract Background: Many studies are in the literature on the ANP and oxytocin-presence in the brain, but very few studies with controversial results are reported on the presence of these peptides in the cerebellum. This immunohistochemical study investigates on the ANP and oxytocin-presence in the cerebellum of the adult rat and mouse rodents. Results: This study, firstly, evidences the ANP- immunopositivity in cerebellar cortex of both rat and mouse rodents. In rat the molecular layer presents some few immunopositive fibers, but no neuron resulted immunopositive; the granular and Purkinje cells are immunopositive. In mouse the cerebellar cortex ANP-immunopositivity is present in all laye…

Athersoclerosis in females patients with rheumatoid arthritis: a two years follow-up

Expression of gelatinases (MMP-2, MMP-9) and cyclooxygenases (COX-1, COX-2) in some benign salivary gland tumors.

Salivary gland tumors, most of which are rare benign tumors, represent a histologically heterogenous group with the greatest diversity of morphological and cellular features. The aim of this study is to analyse the expression and possible interactions between gelatinases (MMP-2, MMP-9) and cyclooxygenases (COX-1, COX-2) in some benign salivary gland tumors. We investigated the expression of gelatinases and cyclooxigenases in control salivary gland, Pleomorphic adenoma and Warthin's tumor through immunohistochemistry and Reverse Transcription – Polymerase Chain Reaction (PCR). We identified the expression of both classes of enzyme in normal samples and in the two types of pathological sampl…

Iniezione locale di infliximab nel trattamento della dattilite

EXPRESSION OF METIOLLOPROTEINASES ( MMP2-MMP9 ) AND CYCLOOXIGENASES ( COX1-COX2 ) IN SALIVARY GLAND TUMORS

Background Salivary neoplasms of the major salivary glands are uncommon: the annual incidence rates in the world vary between greater than 0.05 and less than 2 per 100,000. Most salivary gland tumours are benign and they represent an histological heterogeneous group with the greatest diversity of morphological and cellular features, in fact, these tumours are characterized by an extremely varied histomorphological phenotype due to combinations of different epitheliomorphic and mesenchymomorphic features. The histogenesis is uncertain but the bicellular theory of origin has been accepted and states that malignant transformation of reserve cell from either the intercalated or excretory duct a…

Atherosclerosis in females patients with rheumatoid arthritis; a two years follow-up.

TWENTY YEARS' HISTORY OF IMMUNOHISTOCHEMICAL MAMMALS DENTAL PULP STUDIES

Presence and interaction in tissues of atrial natriuretic peptide,oxytocin and vasopressin:new insights.

Atrial natriuretic peptide, oxytocin and vasopressin are three well known and widely studied molecules since many years. They have been fully characterised from a genetic and biomolecular point of view and a number of receptor-dependent functions have been recognised for them. Nevertheless, in the last years our group has conducted morphologic studies, using an immunohistochemical approach complemented by molecular biology techniques, and could show non-canonical localization and co-localization of these peptides in normal and pathologic tissues, that permitted us to postulate that they may be involved in a wider range of functions than usually assumed and not yet fully understood. In this …

Artropatia sclerodermica ed artrite reumatoide non evidenza di overlap

Imaging ecografico del cordone ombelicale umano.

B-Endorphin in Human Dental Pulp During Orthodontic Treatments

Imaging ecografico del cordone ombelicale umano Ecographic imaging of the human umbilical cord

Presence in trigeminal ganglia in developing rat.

DIASTOLIC DYSFUNCTION IN PATIENTS WITH RHEUMATOID ARTHRITIS TREATED WITH TNF BLOCKING AGENTS

ANP (Atrial Natriuretic Peptide) presence in the heart of a tunicate, Ciona intestinalis.

Atrial natriuretic peptide was found in the heart of vertebrates, we studied the ANP presence in the heart of Ciona intestinalis. This is animal is very important because of the its evolutionary position between invertebrates and vertebrates. ANP presence was only revealed in myoepithelial cells of the myocardium. Results suggest the hypothesis that ANP is present not only in the vertebrates but also in the invertebrates and in Ciona heart ANP might play a similar role like in the heart of vertebrates.

Immunohistochemical and transcriptional expression of the matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa

The oral cavity is exposed to chronic or recurrent, physical and chemical trauma that could lead to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). The objective of this study is to investigate the expression and the possible changes of the two matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa samples. Normal oral mucosa samples and three different types of pathological conditions (hyperplasia, dysplasia and carcinoma) were used for this study. Immunohistochemical analysis was used to evaluate protein expression for the two enzymes, while Reverse Transcription ? Polymerase Chain Reaction (RT-PCR) was used to investigate gene expression. Image…

ANP and vasopressin -presence in the ciliary body of eye in the pig, (Sus domesticus)

The aqueous humor is produced in the ciliary body so, in this study we investigate on the ANP presence and vasopressin in the ciliary body of the pig eye since these peptide are involved in the homeostasis of the body fluids. The results show ANP-presence in the epithelial cells and in the endothelial cells of the blood vessels and VP-presence in the epithelial cells, in the endothelium of canal of Schelmm and in the muscle cells of the blood vessels. These peptides might regulate the synthesis and the composition of the aqueous humor and regulate the hydrodynamic flow and haemodynamic flow of the blood.

Atherosclerosis in female patients with rheumatoid arthritis: a two years of follow up

Disfunzione diastolica in pazienti affetti da artrite reumatoide: ruolo della terapia con anti-TNFα

Impatto del trattamento precoce con infliximab + methotrexate ed alte dosi di prednisone (PDN) vs infliximab (IFX) + methotrexate (MTX) e basse dosi di steroide nella very early rheumatoid arthritis (VERA): contributo casistico

Fattori prognostici e predittivi della early arthritis

Expression of remodelling enzymes (MMP-2, MMP-8, MMP-9) in mixed tumors of salivary gland

Rapida induzione di remissione di un caso di malattia di Vogt Koyanagi Harada recidivante dopo terapia con Infliximab

Twenty years' history of immunohistochemical mammals dental pulps studies.

Functional Aspects of Adrenocholinergic and Peptidergic Innervation about Prostate and Seminal Vesicles in Mammals: A Review

This study was aimed to explain the functional role of mediation in the organs of the male reproductive system (prostate and seminal vesicles of rat and hamster), representing throught the visualization of adrenergic fibres (Falck Method), cholinergic fobres (Martinez-Rodriguez method) and by immunohistochemical methods to detect the P substance and the CGRP (PAP) in glandular structures. The adrenocholinergic and peptidergic mediators have a growing interest for their synergic action in the control of contractility, vasodilatation and most likely, of the secretory activity of the epithelium, probably expressed by andocrine-paracrine mechanisms.

Expression of Remodelling Enzymes (MMP-2, MMP-9) in Normal and Pathological Oral Mucosa Samples

Introduction. The oral cavity contains many structures compressed in a small area. It is exposed to chronic or recurrent, physical and chemical trauma leading to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). For a long time, our group has been studying the expression of some metalloproteinases (MMP-2, MMP-9) in healthy and pathological human oral mucosa through immunohistochemistry and RT-PCR to investigate the entity of the remodelling process in the different cases. Matherials and Methods. In this paper we investigate a sample of normal oral mucosa and four pathological samples (mucocele, fibroma, dysplasia and carcinoma). We identified the localization of MMP-2 and MMP-9 th…

The level of solubile granzyme A is elevated in the plasma and in the Vγ9/Vδ2 T cell culture supernatants of patients with active Behçet's disease.

Fattori prognostici e predittivi della early arthritis (EA)

Atherosclerosis in female patients with rheumatoid arthritis; a two years follow-up

Expression of Remodelling Enzymes MMPs in Hernia Formation

ANP Presence in trigeminal ganglia in developing rat

Vent'anni di storia sullo studio immunoistochimico delle polpe dentali di mammiferi Twenty years'history of immunohistochemical mammals dental pulp studies

Impatto del trattamento precoce con infliximab + methotrexate (MTX) ed alte dosi di prednisone (PDN) vs infliximab (IFX)+methotrexate (MTX) e basse dosi di steroide nella very early rheumatoid arthritis (VERA): contributo casistico

Oxytocin and vasopressin expression in the turbinates of patients with chronic sinusitis

Many peptides are present in the nasal mucosa, but few studies have investigated the presence or absence of the oxytocin and vasopressin peptides. This immunohistochemical study on the inferior turbinates of patients affected by chronic sinusitis shows, for the first time, that these peptides are present in the epithelium of both nasal mucosa and glands. Their presence could be related to the presence of atrial natriuretic peptide (ANP), like previously demonstrated in other organs such as heart and prostate, since in some circumstances they play in antagonism.