0000000001186402
AUTHOR
V. Mondello
Pathogenicity and possible biological control of fungi associated to Botryosphaeria dieback of grapevine in Western Sicily
Botryosphaeria dieback is a widespread grapevine trunk disease caused by species of fungi within the family Botryosphaeriaceae. Symptoms of the disease include bud necrosis, leaf chlorosis, vascular discoloration of the wood, cankers and dieback. In 2013 typical symptoms of Botryosphaeria dieback was observed in Western Sicily and four species associated with the syndrome were isolated and identified as Diplodia seriata, Lasiodiplodia mediterranea, Neofusicoccum parvum and Neofusicoccum vitifusiforme. In order to fulfill Koch’s postulates, pathogenicity tests of eighteen isolates were performed on two-year grapevine cuttings of cv. Inzolia. Six months after inoculation all the Botryosphaeri…
Interaction between Acremonium byssoides and Plasmopara viticola in Vitis vinifera
The endophytic fungus Acremonium byssoides was isolated from the leaves of grapevine cv. Regina Bianca to determine whether it could act as an antagonistic endophyte of the downy mildew agent Plasmopara viticola. The occurrence of this fungus was ascertained in 34 grapevines (cultivars Regina Bianca, Catarratto and Insolia) representing all the grapevine plants in an experimental vineyard located in Sicily and which had never been treated with any fungicides. The isolation frequency of the endophytic Acremonium strains was assessed in several plant organs by means of monthly samplings over a 2-year period. The endophyte was found in all the grapevines, with higher frequencies in the cv. Reg…
Les maladies du bois de la vigne : recherches pour apporter des solutions de lutte aux Professionnels
Les travaux réalisés sur deux maladies du bois de la vigne (esca, BDA) ont montré leur effet sur la récolte et la croissance de la pousse printanière qui était annulé deux années après l’apparition des symptômes. Ces maladies ont aussi conduit à des modifications des propriétés analytiques des moûts et des vins. Les vins issus de raisins collectés sur des ceps symptomatiques n’étaient pas toujours les plus dépréciés. Les études effectuées sur le cycle biologique ont permis de mettre au point des outils pour différencier les individus de deux espèces de Botryosphaeriaceae et des outils d’hybridation in situ qui permettront de les localiser précisément dans les tissus. Elles ont aussi identif…
Endophytic Fungi in Asymptomatic Vitis vinifera L. and their Effects on Plasmopara viticola
During a multi-year study on the life cycle of Plasmopara viticola to assess the typical epidemiology of downy mildew on grapevine (Vitis vinifera) in west Sicily (Italy), several leaves on which the downy mildew had collapsed were found and observed in a vineyard located in Marsala, in Trapani Province. An antagonistic fungus was isolated from these leaves and named A20; through its morphology, it was identified as a hyphomycete belonging to the genus Acremonium. The antagonism of A20 against the asexual and sexual spores of P. viticola, as well as endophytic nature of the genus Acremonium in V. vinifera were confirmed by further studies which have been carried out over the past decade. In…
Grapevine decline in Italy caused by Lasiodiplodia theobromae
The first report of a dieback of grapevine caused by Lasiodiplodia theobromae in Sicily (Italy) is given. About twelve per cent of the vines in the cv. Insolia vineyard surveyed, showed spur dieback, retarded growth and wood necrosis. Isolation trials and pathogenicity tests are briefl y reported, together with morphological, cultural and molecular characters on which identification was based.
First report of Neofusiccoccum vitifusiforme and presence of other Botryosphaeriaceae species associated with Botryosphaeria dieback of grapevine in Sicily (Italy)
Summary. Since 2007, when a grapevine decline caused by Lasiodiplodia theobromae was reported for the first time in Sicily, vines showing similar declining symptoms have been also found in other grape-growing areas of western and central Sicily. We report the result of a study on fungi associated with grapevine decline in Sicily, with particular regard to those belonging to the Botryosphaeriaceae. Four species were found to be associated with declining vines, namely Diplodia seriata, Lasiodiplodia sp., Neofusicoccum parvum and Neofusicoccum vitifusiforme, the latter species reported for the first time on Vitis vinifera in Italy.