Die direkte quantitative auswertung von dünnschichtchromatogrammen durch remissions- und fluoreszenzmessungen

Abstract Direct quantitative evaluation of thin-layer chromatograms by remission- and fluorescence measurements. Part 6. Determination of adrenaline, noradrenaline and dopamine from urine Adrenaline, noradrenalineand dopamine were separated from urine on aluminium oxide columns; after elution they were converted into ther O 3 ,O 4 ,N-triacetyl derivatives and separated using thin-layer chromatography. The triacetyl derivatives were in situ converted to fluorescing compounds using ethylene diamine and quantitatively estimated using the Chromatogram-Spectrophotometer (Carl Zeiss, Oberkochen). The advantage of this method over the conventional fluorometric determination of catecholamines in so…