BIO-PULITURA: PROTEASI DA ORGANISMI MARINI.

Titolo: Novel proteases from marine organisms with potential interest in restoration procedure

3D Collagen matrix improves islets of Langerhans differentiation and function

The importance of preserve cell-cell contact and cell-matrix interaction are both fundamental for maintain the differentiation state. We use both standardized method of isolation and culture in 3D matrix which improves islet cell survival and function for more than one week in colture.

COLLAGENASI RICOMBINANTI DI C. HISTOLYTICUM E METODO PER LA LORO PRODUZIONE.

La presente descrizione si riferisce alla produzione di collagenasi ricombinanti, in particolare è qui descritto un metodo per la produzione di collagenasi Col ricombinante di Clostridium histolyticum caratterizzato da una resa maggiore a circa 140mg/l di coltura di detta collagenasi in forma biologicamente attiva, collagenasi prodotte mediante detto metodo, composizioni che le comprendono e loro uso.

Tissue Dissociation and Primary Cells Isolation Using Recombinant Collagenases Class I and II

Collagenases class I (Col G) and class II (Col H) currently available for tissue dissociation are produced from Clostridium histolyticum (human pathogen) strains. In the processes of extraction of the cells from the tissue, combined activity of both classes of enzymes is required. CI and CII are complementary in degrading collagen. ABIEL recently produced the collagenase class I and II using the recombinant DNA technologies (PCT WO 2011/073925 A9). The enzymes were produced in E. coli and purified by affinity chromatography. The method of production adopted allows absolute control of the final composition of these enzymes, as well as their stability, purity, activity, absence of toxicity an…

C. HYSTOLITICUM RECOMBINANT COLLAGENASES AND METHOD FOR THE MANUFACTURE THEREOF

The present invention relates to the production of recombinant collagenases, and in particular describes a method for the production of recombinant clostridium histolyticum collagenases Col characterized by a yield higher than approximately 140 mg/l of culture of said collagenases in soluble, biologically active form, collagenases produced by this method, compositions comprising these collagenases and the use thereof.

CLUSTERING OF TYPE-II TRANSMEMBRANE SERINE PROTEASES (TTSPS) AND MATRIX METALLOPROTEINASES (MMPS) IN THE CELL SURFACE AND SHED MEMBRANE VESICLES OF ACTIVE ENDOTHELIAL CELLS.

Overespression of MT1-MMP modified the integrity of endothelial cell contact

Clustering of type-II transmembrane serine proteases (TTSPs) and matrix metalloproteinases (MMPs) in the cell surface and shed membrane vesicles of active endothelial cells.

Removal of old protein layers from surfaces of works of art by new enzymes

The aim of the present project is to set up bio-cleaning protocols in order to easily remove altered protein layers (e.g. animal glues) by enzymatic proteins (proteases). Protein molecules were isolated from marine invertebrate organisms, characterized by size exclusion highpressure liquid chromatography (Waters: SEC-HPLC, BioSuite 250 to 10㎛ SEC 7.5 x 300 mm) and their gelatinase activity analyzed by zymography on polyacrilamide gel. The remarkably proteolytic activity and the reaction temperature range of these enzymes, 4° to 37℃, made it possible to hypothesize their use in bioremediation projects. Tests were performed on protein coating found on some polychrome wooden artifacts exposed …

Novel proteases from marine organisms with potential interest in restoration procedure

In the last decades, molecular biology allowed the development of innovative protocols in the field of conservation/restoration of cultural assets. In this work new hydrolyses, isolated from marine invertebrate organisms, are applied to remove protein layers form works of art surface. Proteolytic zymography assay evidenced that these enzymes are active in a broad temperature range, between 4 degrees and 37 degrees C. The enzymatic cleaning by these proteases, tested on wooden furniture of the second half of the eighteenth century showed positive results, without needing to heat the enzyme solution or the surface on which they were applied. The present report proposes novel proteases more ap…