0000000001232802

AUTHOR

Anna Maria Cerio

showing 2 related works from this author

Transplantation of low dose CD34+KDR+ cells promotes vascular and muscular regeneration in ischemic limbs.

2004

Hematopoietic progenitor cell transplantation can contribute to revascularization of ischemic tissues. Yet, the optimal cell population to be transplanted has yet to be determined. We have compared the therapeutic potential of two subsets of human cord blood CD34+ progenitors, either expressing the VEGF-A receptor 2 (KDR) or not. In serum-free starvation culture, CD34+KDR+ cells reportedly showed greater resistance to apoptosis and ability to release VEGF-A, as compared with CD34+KDR- cells. When injected into the hind muscles in immunodeficient SCIDbg mice subjected to unilateral ischemia, a low number (10(3)) of CD34+KDR+ cells improved limb salvage and hemodynamic recovery better than a …

Pathologymedicine.medical_specialtyAngiogenesismedicine.medical_treatmentPopulationMuscle Fibers SkeletalIschemiaNeovascularization PhysiologicAntigens CD34ApoptosisRevascularizationBiochemistryMiceIschemiaGeneticsmedicineAnimalsHumansRegenerationeducationMuscle SkeletalMolecular Biologyeducation.field_of_studybusiness.industryRegeneration (biology)Stem CellsHemodynamicsKinase insert domain receptorExtremitiesmedicine.diseaseFetal BloodFibrosisVascular Endothelial Growth Factor Receptor-2TransplantationImmunologyStem cellbusinessBiotechnologyStem Cell TransplantationFASEB journal : official publication of the Federation of American Societies for Experimental Biology
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Human Haemato-Endothelial Precursors: Cord Blood CD34+ Cells Produce Haemogenic Endothelium

2012

Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports the presence of haemogenic endothelium and haemangioblasts in later stages of development. Our studies indicate that human cord blood haematopoietic progenitors (CD34+45+144-), triggered by murine hepatocyte conditioned medium, differentiate into adherent proliferating endothelial precursors (CD144+CD105+CD146+CD31+CD45-) capable of functioning as haemogenic endothelium. These cells, proven to give…

CD31MouseCellular differentiationMESH: HematopoiesisAntigens CD34murine hepatocytesMESH: CadherinsMESH: HepatocytesMice0302 clinical medicineMolecular Cell BiologyHematopoiesiHepatocyteMESH: Animalsendothelial lineageMESH: Antigens CDCells Cultured0303 health sciencesMultidisciplinaryMESH: Culture Media ConditionedStem CellsMedicine (all)QMESH: Infant NewbornRMESH: HemangioblastsAntigens CD45Cell DifferentiationAnimal ModelsCadherinsFetal BloodCell biologyAdult Stem CellsHaematopoiesisPhenotypeconditioned mediummedicine.anatomical_structureCord bloodMedicineHemangioblastCD146Cellular TypesAnimals; Antigens CD; Antigens CD34; Antigens CD45; Cadherins; Cell Adhesion; Cell Differentiation; Cell Shape; Cells Cultured; Culture Media Conditioned; Fetal Blood; Hemangioblasts; Hematopoiesis; Hepatocytes; Humans; Immunophenotyping; Infant Newborn; Mice; Phenotype; Agricultural and Biological Sciences (all); Biochemistry Genetics and Molecular Biology (all); Medicine (all)Research ArticleHumanMESH: Cells Culturedendothelial lineage; murine hepatocytes; conditioned mediumMESH: Cell DifferentiationMESH: ImmunophenotypingEndotheliumHemangioblastsScienceMESH: Antigens CD45[SDV.BC]Life Sciences [q-bio]/Cellular BiologyBiologyMESH: PhenotypeImmunophenotypingMESH: Cell Adhesion03 medical and health sciencesModel OrganismsAntigens CDCell AdhesionmedicineAnimalsHumansMESH: Cell ShapeMESH: Fetal BloodProgenitor cellBiologyCell ShapeMESH: Mice030304 developmental biologyBiochemistry Genetics and Molecular Biology (all)MESH: HumansAnimalInfant NewbornMESH: Antigens CD34Hematopoietic Stem CellsHemangioblastHematopoiesisAgricultural and Biological Sciences (all)Culture Media ConditionedImmunologyHepatocytesCadherinLeukocyte Common Antigens030217 neurology & neurosurgeryDevelopmental BiologyPLoS ONE
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