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RESEARCH PRODUCT
Human Haemato-Endothelial Precursors: Cord Blood CD34+ Cells Produce Haemogenic Endothelium
Laura AmiconeMarco De Santis PuzzoniaGermana CastelliVeronica BordoniMauro BiffoniElvira PelosiAnna Maria CerioFrancesco BertoliniUgo TestaTonino AlonziPaola MarighettiMarco TripodiMyriam AlcalayAlice ConigliaroInes Martin-paduraSimona Santorosubject
CD31MouseCellular differentiationMESH: HematopoiesisAntigens CD34murine hepatocytesMESH: CadherinsMESH: HepatocytesMice0302 clinical medicineMolecular Cell BiologyHematopoiesiHepatocyteMESH: Animalsendothelial lineageMESH: Antigens CDCells Cultured0303 health sciencesMultidisciplinaryMESH: Culture Media ConditionedStem CellsMedicine (all)QMESH: Infant NewbornRMESH: HemangioblastsAntigens CD45Cell DifferentiationAnimal ModelsCadherinsFetal BloodCell biologyAdult Stem CellsHaematopoiesisPhenotypeconditioned mediummedicine.anatomical_structureCord bloodMedicineHemangioblastCD146Cellular TypesAnimals; Antigens CD; Antigens CD34; Antigens CD45; Cadherins; Cell Adhesion; Cell Differentiation; Cell Shape; Cells Cultured; Culture Media Conditioned; Fetal Blood; Hemangioblasts; Hematopoiesis; Hepatocytes; Humans; Immunophenotyping; Infant Newborn; Mice; Phenotype; Agricultural and Biological Sciences (all); Biochemistry Genetics and Molecular Biology (all); Medicine (all)Research ArticleHumanMESH: Cells Culturedendothelial lineage; murine hepatocytes; conditioned mediumMESH: Cell DifferentiationMESH: ImmunophenotypingEndotheliumHemangioblastsScienceMESH: Antigens CD45[SDV.BC]Life Sciences [q-bio]/Cellular BiologyBiologyMESH: PhenotypeImmunophenotypingMESH: Cell Adhesion03 medical and health sciencesModel OrganismsAntigens CDCell AdhesionmedicineAnimalsHumansMESH: Cell ShapeMESH: Fetal BloodProgenitor cellBiologyCell ShapeMESH: Mice030304 developmental biologyBiochemistry Genetics and Molecular Biology (all)MESH: HumansAnimalInfant NewbornMESH: Antigens CD34Hematopoietic Stem CellsHemangioblastHematopoiesisAgricultural and Biological Sciences (all)Culture Media ConditionedImmunologyHepatocytesCadherinLeukocyte Common Antigens030217 neurology & neurosurgeryDevelopmental Biologydescription
Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports the presence of haemogenic endothelium and haemangioblasts in later stages of development. Our studies indicate that human cord blood haematopoietic progenitors (CD34+45+144-), triggered by murine hepatocyte conditioned medium, differentiate into adherent proliferating endothelial precursors (CD144+CD105+CD146+CD31+CD45-) capable of functioning as haemogenic endothelium. These cells, proven to give rise to functional vasculature in vivo, if further instructed by haematopoietic growth factors, first switch to transitional CD144+45+ cells and then to haematopoietic cells. These results highlight the plasticity of haemato-endhothelial precursors in human post-natal life. Furthermore, these studies may provide highly enriched populations of human post-fetal haemogenic endothelium, paving the way for innovative projects at a basic and possibly clinical level. © 2012 Pelosi et al.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-01-01 | PLoS ONE |