0000000001300449
AUTHOR
Francesco Saverio Pavone
showing 6 related works from this author
Power-effective scanning with AODs for 3D optogenic applications
2022
Two-photon (2P) excitation is a cornerstone approach widely employed in neuroscience microscopy for deep optical access and sub-micrometric-resolution light targeting into the brain. However, besides structural and functional imaging, 2P optogenetic stimulations are less routinary, especially in 3D. This is because of the adopted scanning systems, often feebly effective, slow and mechanically constricted. Faster illumination can be achieved through acousto-optic deflectors (AODs) although their applicability to large volumes excitation has been limited by large efficiency drop along the optical axis. Here, we present a new AOD-based scheme for 2P 3D scanning that improves the power delivery…
A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy
2023
The analysis of histological alterations in all types of tissue is of primary importance in pathology for highly accurate and robust diagnosis. Recent advances in tissue clearing and fluorescence microscopy made the study of the anatomy of biological tissue possible in three dimensions. The combination of these techniques with classical hematoxylin and eosin (H&E) staining has led to the birth of three-dimensional (3D) histology. Here, we present an overview of the state-of-the-art methods, highlighting the optimal combinations of different clearing methods and advanced fluorescence microscopy techniques for the investigation of all types of biological tissues. We employed fluorescence …
Visualization_1
2022
Volumetric recording of a single CRIW event shown as a selected subset of coronal sections. To produce the time lapse, original 16-bit depth images were converted into 8-bit and JPEG compressed. Scale bar: 100 ��m.
Visualization_2
2022
3D rendering of the lag map shown in Fig. 4a. The lag value is color-coded as specified by the color bar. Scale bar: 100 ��m.
Visualization_1
2022
Volumetric recording of a single CRIW event shown as a selected subset of coronal sections. To produce the time lapse, original 16-bit depth images were converted into 8-bit and JPEG compressed. Scale bar: 100 ��m.
Visualization_2
2022
3D rendering of the lag map shown in Fig. 4a. The lag value is color-coded as specified by the color bar. Scale bar: 100 ��m.