0000000001308366

AUTHOR

Karl Wandner

showing 3 related works from this author

Plasmonic Nanosensors for the Label-Free Imaging of Dynamic Protein Patterns.

2020

We introduce a new approach to monitor the dynamics and spatial patterns of biological molecular assemblies. Our molecular imaging method relies on plasmonic gold nanoparticles as point-like detectors and requires no labeling of the molecules. We show spatial resolution of up to 5 μm and 30 ms temporal resolution, which is comparable to wide-field fluorescence microscopy, while requiring only readily available gold nanoparticles and a dark-field optical microscope. We demonstrate the method on MinDE proteins attaching to and detaching from lipid membranes of different composition for 24 h. We foresee our new imaging method as an indispensable tool in advanced molecular biology and biophysic…

Materials scienceCardiolipinsLipid BilayersMetal NanoparticlesNanotechnologyCell Cycle Proteins02 engineering and technology010402 general chemistry01 natural sciencesNanosensorFluorescence microscopeEscherichia coliGeneral Materials SciencePhysical and Theoretical ChemistryImage resolutionPlasmonAdenosine TriphosphatasesMicroscopyNanotubesEscherichia coli ProteinsPhosphatidylglycerols021001 nanoscience & nanotechnology0104 chemical sciencesMembraneColloidal goldTemporal resolutionPhosphatidylcholinesGoldMolecular imaging0210 nano-technologyThe journal of physical chemistry letters
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Intensity-Based Single Particle Plasmon Sensing.

2021

Plasmon sensors respond to local changes of their surrounding environment with a shift in their resonance wavelength. This response is usually detected by measuring light scattering spectra to determine the resonance wavelength. However, single wavelength detection has become increasingly important because it simplifies the setup, increases speed, and improves statistics. Therefore, we investigated theoretically how the sensitivity toward such single wavelength scattering intensity changes depend on the material and shape of the plasmonic sensor. Surprisingly, simple equations describe this intensity sensitivity very accurately and allow us to distinguish the various contributions: Rayleigh…

PhysicsScatteringbusiness.industryMechanical EngineeringPhysics::OpticsBioengineering02 engineering and technologyGeneral Chemistry021001 nanoscience & nanotechnologyCondensed Matter PhysicsLight scatteringSpectral linesymbols.namesakeWavelengthOpticssymbolsGeneral Materials ScienceRayleigh scattering0210 nano-technologySpectroscopybusinessIntensity (heat transfer)PlasmonNano letters
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Plasmonic Nanosensors for the Label-Free Imaging of Dynamic Protein Patterns

2020

Additional data to support our work on "Plasmonic Nanosensors for the Label-Free Imaging of Dynamic Protein Patterns" published in the Journal of Physical Chemistry Letters (DOI: 10.1021/acs.jpclett.0c01400) Movies: - S1: MinVideo_EColi.mp4 - S2: MinVideo_DOPC_DOPG_CL.mp4 - S3: MinVideo_DOPC_DOPG.mp4 Audio Files: - S1: MinSound_EColi.mp4 - S2: MinSound_DOPC_DOPG_CL.mp4 - S3: MinSound_DOPC_DOPG.mp4

Label-free NanosensorsProtein PatternsMinDE OscillationsMolecular Imaging
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