6533b855fe1ef96bd12afda1
RESEARCH PRODUCT
Plasmonic Nanosensors for the Label-Free Imaging of Dynamic Protein Patterns.
Rubén Ahijado-guzmánKatharina KaeferCarsten SönnichsenKarl WandnerFelix SchlappWeixiang YeSirin Celiksoysubject
Materials scienceCardiolipinsLipid BilayersMetal NanoparticlesNanotechnologyCell Cycle Proteins02 engineering and technology010402 general chemistry01 natural sciencesNanosensorFluorescence microscopeEscherichia coliGeneral Materials SciencePhysical and Theoretical ChemistryImage resolutionPlasmonAdenosine TriphosphatasesMicroscopyNanotubesEscherichia coli ProteinsPhosphatidylglycerols021001 nanoscience & nanotechnology0104 chemical sciencesMembraneColloidal goldTemporal resolutionPhosphatidylcholinesGoldMolecular imaging0210 nano-technologydescription
We introduce a new approach to monitor the dynamics and spatial patterns of biological molecular assemblies. Our molecular imaging method relies on plasmonic gold nanoparticles as point-like detectors and requires no labeling of the molecules. We show spatial resolution of up to 5 μm and 30 ms temporal resolution, which is comparable to wide-field fluorescence microscopy, while requiring only readily available gold nanoparticles and a dark-field optical microscope. We demonstrate the method on MinDE proteins attaching to and detaching from lipid membranes of different composition for 24 h. We foresee our new imaging method as an indispensable tool in advanced molecular biology and biophysics laboratories around the world.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-05-21 | The journal of physical chemistry letters |