6533b7cefe1ef96bd12576d6

RESEARCH PRODUCT

Protection of yeast cells in micro-organized shells of natural polyelectrolytes during drying process

subject

description

The encapsulation techniques are applied in various fields and for various applications. The layer-by-layer self-assembly (LbL), one of the encapsulation techniques, is built by the electrostatic attraction between oppositely charged polyelectrolytes, and the topmost layer determines essentially the sur-face properties of the edifice. This technique offers several advantages (low cost, simplicity of process and equipment, biocompatibility and biodegra-dation, etc.). In this present paper, results of the protection of Saccharomyces cerevisiae yeast cells in microorganized shell of natural polyelectrolytes during dehydration process are reported. To apply the LbL method to individually encapsulated cells, β-lactoglobulin and sodium alginate were used as polycation and polyanion. The protective effect against dehydration was evaluated by the measure of cell survival. Moreover, we explored the potential-ity of the Fourier transform infrared microspectros-copy with the synchrotron beam source (S-FTIR) in order to analyze biochemical composition changes of S. cerevisiae at single-cell level.