Heat shock response in yeast involver changes in both transcription rates and mRNA stabilities

6533b7cefe1ef96bd1257a42

RESEARCH PRODUCT

Heat shock response in yeast involver changes in both transcription rates and mRNA stabilities

José E. Pérez-ortín Joaquín Moreno José García-martínez Enrique Herrero Gemma Bellí Laia Castells-roca

subject

Llevat de cervesa Transcription Genetic Estrès oxidatiu RNA Stability Saccharomyces cerevisiae Gene Expression lcsh:Medicine Yeast and Fungal Models RNA-binding protein Saccharomyces cerevisiae Models Biological Genètica molecular Model Organisms Transcripció genètica Genome Analysis Tools Transcription (biology)Gene Expression Regulation Fungal Yeasts Heat shock protein Molecular Cell Biology Genetics Cluster Analysis RNA Messenger Heat shock lcsh:Science Biology Gene Transcription factor Heat-Shock Proteins Multidisciplinary Base Sequence Organisms Genetically Modified biology Systems Biology lcsh:R RNA Fungal Llevats -- Genètica Genomics biology.organism_classification Molecular biology Functional Genomics Cell biology Regulon RNA lcsh:Q Genome Expression Analysis Heat-Shock Response Research Article Transcription Factors

description

We have analyzed the heat stress response in the yeast Saccharomyces cerevisiae by determining mRNA levels and transcription rates for the whole transcriptome after a shift from 25uC to 37uC. Using an established mathematical algorithm, theoretical mRNA decay rates have also been calculated from the experimental data. We have verified the mathematical predictions for selected genes by determining their mRNA decay rates at different times during heat stress response using the regulatable tetO promoter. This study indicates that the yeast response to heat shock is not only due to changes in transcription rates, but also to changes in the mRNA stabilities. mRNA stability is affected in 62% of the yeast genes and it is particularly important in shaping the mRNA profile of the genes belonging to the environmental stress response. In most cases, changes in transcription rates and mRNA stabilities are homodirectional for both parameters, although some interesting cases of antagonist behavior are found. The statistical analysis of gene targets and sequence motifs within the clusters of genes with similar behaviors shows that both transcriptional and post-transcriptional regulons apparently contribute to the general heat stress response by means of transcriptional factors and RNA binding proteins.

year	journal	country	edition	language
2011-01-01

10.1371/journal.pone.0017272 http://hdl.handle.net/10550/31672