6533b7cefe1ef96bd1257bae

RESEARCH PRODUCT

Immunoperoxidase Staining of Fine-Needle Aspiration Biopsies of Renal Cell Carcinoma Using Tumor-Specific Monoclonal Antibody

Peter AlkenMasayoshi YokoyamaT. SchärfeRudolf HohenfellnerGünter H. Jacobi

subject

AdenomaPathologymedicine.medical_specialtymedicine.drug_classUrologymedicine.medical_treatmenturologic and male genital diseasesMonoclonal antibodyImmunoenzyme TechniquesAntigens NeoplasmRenal cell carcinomaBiopsymedicineHumansCarcinoma Renal CellFrozen section proceduremedicine.diagnostic_testImmunoperoxidasebusiness.industryBiopsy NeedleAntibodies Monoclonalmedicine.diseaseKidney Neoplasmsfemale genital diseases and pregnancy complicationsNephrectomyStainingFine-needle aspirationbusiness

description

In 30 nephrectomy specimens, fine needle aspiration biopsies (FNAB) were performed both in the tumor and in the macroscopically normal part of the kidney: 18 were well differentiated renal cell carcinoma (RCC), 6 were moderately differentiated RCC, 4 were poorly differentiated RCC, and 2 were oncocytomas. FNAB was also performed in a bone metastasis of RCC. FNAB materials were stained using the immunoperoxidase method with RCC-specific monoclonal antibody and were compared with the staining of frozen sections. In all cases where tumor-antigen expression could be demonstrated in the frozen sections, a FNAB had already proven positive. There were only 2 false-negative cases where sufficient cellular material could not be obtained due to extensive tumor necrosis. There was no false-positive case. Oncocytomas showed no expression of antigen. The use of immunoperoxidase staining of FNAB with tumor-specific monoclonal antibody is discussed.

yearjournalcountryeditionlanguage
1987-01-01European Urology
https://doi.org/10.1159/000472813