6533b7cffe1ef96bd1258483
RESEARCH PRODUCT
Naturally occurring hemolysins in the coelomic fluid of Holothuria polii delle chiaie (Echinodermata).
Nicolò ParrinelloCalogero CanicattìDaniela Rindonesubject
ErythrocytesCations DivalentSea CucumbersImmunologyDose-Response Relationship Immunologicchemistry.chemical_elementCalciumHemolysisHemolysin ProteinsPotencyAnimalsbiologyLabilityProteolytic enzymesTemperatureHemolysinExudates and TransudatesHydrogen-Ion Concentrationbiology.organism_classificationKineticschemistryBiochemistryCoelomAbsorption (chemistry)HolothuriaDevelopmental BiologyEchinodermatadescription
Abstract The coelomic fluid of Holothuria polii D.Ch contains hemolytic activity against erythrocytes of several vertebrate species. The hemolytic potency depends upon calcium ion concentration and varies according to erythrocyte source and cell number in the reaction mixture. Absorption experiments with formalinized rabbit erythrocytes suggest that hemolytic activity is not specific. Its heat lability, water insolubility at low pH values, and sensitivity to proteolytic enzymes suggest that hemolytic activity resides in protein molecules. The activity, maximal in alkaline media, appears to depend up time and temperature.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1979-01-01 | Developmental and comparative immunology |