Ar jauniem bioloģiskiem preparātiem apstrādātās augsnes molekulāri bioloģiskais raksturojums

6533b7cffe1ef96bd125937b

RESEARCH PRODUCT

Ar jauniem bioloģiskiem preparātiem apstrādātās augsnes molekulāri bioloģiskais raksturojums

Māris Seņkovs

subject

ARDRA PCR Bioloģija bioloģiskie preparāti augsne

description

Šī pētījuma mērķis bija noskaidrot divu jaunu bioloģisko preparātu ietekmi uz augsnes mikroorganismu ģenētiskā materiāla koncentrācijām un sugu sastāvu. Pētījums tika sadalīts divos neatkarīgos eksperimentos. Pirmajā eksperimentā pētāmie augsnes parauglaukumi tika apstrādāti ar mikroorganismus saturošu kūdras preparātu, bet otrajā – rapša salmiem un mikroorganismiem ar celulolītisko aktivitāti. Parauglaukumos audzēja miežus un rapsi. Pētījumā tika pielietotas gan klasiskās mikrobioloģijas metodes, lai identificētu augsnes mikrobiotas raksturīgākos pārstāvjus, gan molekulārās – PCR, qPCR, ARDRA, lai noteiktu augsnes mikroorganismu ģenētiskā materiāla koncentrācijas. Tika veikta klasteranalīze paraugu ģenētiskā profila salīdzināšanai. Abi pētītie jaunie bioloģiskie preparāti ietekmēja mikroorganismu koncentrāciju miežu un rapša augsnē, un atšķirīgos veidos izmainīja mikrobiotas sugu sastāvu. Augsnes paraugos dominēja nepatogēnu sugu baktērijas: Bacillus mycoides, Brevibacillus brevis, Brevundimonas vesicularis un Sphingomonas paucimobilis.

year	journal	country	edition	language
2017-01-01

https://dspace.lu.lv/dspace/handle/7/35174

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