Trichoderma asperellum, Bacillus subtilis un to konsorcija ietekme uz augsnes mikroorganismiem

6533b7d0fe1ef96bd1259dcd

RESEARCH PRODUCT

Trichoderma asperellum, Bacillus subtilis un to konsorcija ietekme uz augsnes mikroorganismiem

Māris Seņkovs

subject

qPCR mikroorganismi mikrobioloģiskie konsorciji KVV Bioloģija augsne

description

Pētījuma mērķis bija noskaidrot, vai Trichoderma asperellum, Bacillus subtilis un to konsorcijs ietekmē augsnes mikrobiotu. Pētījumā eksperimentālie augsnes paraugi tika apstrādāti ar B. subtilis, T.asperellum un B. cinerea mikroorganismiem un to konsorcijiem. Izmantojot mikrobioloģiskās metodes, tika noteikts baktēriju un sēņu daudzums dažādi apstrādātās augsnēs. Ar Biolog EcoPlates metodi, noteikta mikroorganismu metaboliskā aktivitāte augsnē. Trichoderma ģints, Firmicutes, Gammaproteobacteria un Acidobacteria DNS koncentrācija augsnes paraugos tika noteikta ar molekulārajām metodēm. Tika veikta korelācijas analīze starp mikrobioloģisko un molekulāro un tikai starp dažādiem molekulāro rezultātu datiem. Augsnes apstrāde ar dažādiem mikroorganismiem un to konsorcijiem kā palielināja, tā arī samazināja noteiktu taksonu kolonijas veidojošo vienību skaitu un amplificēto DNS fragmentu daudzumu augsnē. Mikroorganismi un to konsorciji palielināja ogļhidrātu un komplekso oglekļa savienojumu izmantošanu, bet samazināja organisko fosfora savienojumu izmantošanu augsnē. Palielinoties KVV/g, palielinājās arī amplificēto DNS fragmentu koncentrācija noteiktos paraugos pēc noteikta dienu skaita.

year	journal	country	edition	language
2019-01-01

https://dspace.lu.lv/dspace/handle/7/47265

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