6533b7d0fe1ef96bd125af57
RESEARCH PRODUCT
Natural hybrids fromSaccharomyces cerevisiae,Saccharomyces bayanusandSaccharomyces kudriavzeviiin wine fermentations
Amparo QuerolJürg GafnerSara S. GonzálezSara S. GonzálezEladio Barriosubject
Nuclear geneGenes FungalSaccharomyces cerevisiaeSaccharomyces bayanusWineSaccharomyces cerevisiaeDNA MitochondrialApplied Microbiology and BiotechnologyMicrobiologySaccharomycesElectron Transport Complex IVSaccharomycesDNA Ribosomal SpacerDNA FungalPhylogenyWineFermentation in winemakingGeneticsbiologySaccharomyces eubayanusGeneral Medicinebiology.organism_classificationElectrophoresis Gel Pulsed-FieldRNA Ribosomal 5.8SKaryotypingFermentationHybridization GeneticPolymorphism Restriction Fragment LengthSaccharomyces kudriavzeviidescription
Several wine isolates of Saccharomyces were analysed for six molecular markers, five nuclear and one mitochondrial, and new natural interspecific hybrids were identified. The molecular characterization of these Saccharomyces hybrids was performed based on the restriction analysis of five nuclear genes ( CAT8 , CYR1 , GSY1 , MET6 and OPY1 , located in different chromosomes), the ribosomal region encompassing the 5.8S rRNA gene and the two internal transcribed spacers, and sequence analysis of the mitochondrial gene COX2 . This method allowed us to identify and characterize new hybrids between Saccharomyces cerevisiae and Saccharomyces kudriavzevii , between S. cerevisiae and Saccharomyces bayanus , as well as a triple hybrid S. bayanus × S. cerevisiae × S. kudriavzevii . This is the first time that S. cerevisiae × S. kudriavzevii hybrids have been described which have been involved in wine fermentation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-12-13 | FEMS Yeast Research |