6533b7d0fe1ef96bd125b8a7
RESEARCH PRODUCT
Karyotypic diversity among Blastocystis hominis isolates
L. Del CastilloJ.a. CarbajalJ. VillarMa.d. LanuzaRafael Borrássubject
GeneticsBlastocystisbiologyGenetic VariationKaryotypeBlastocystis InfectionsDNA ProtozoanLoboseabiology.organism_classificationChromosomesElectrophoresis Gel Pulsed-FieldInfectious DiseasesHomogeneousKaryotypingBlastocystis InfectionsGenetic variationAnimalsCluster AnalysisHumansParasite hostingBlastocystis hominisParasitologyAxenicdescription
Fifteen Blastocystis hominis strains, 13 axenic and 2 monoxenic, have been included in the present study. The chromosomal pattern was analyzed by the contour-clamped homogeneous electric-field (CHEF) system. The number of chromosomes detected ranged between 9 and 13, with sizes from 2200 kbp to 260 kbp. Eleven karyotypic profiles, with a common pattern constituted by 8 chromosomes of 2200, 1280, 890, 840, 700, 650, 540 and 260 kbp, were observed. The Jaccard index demonstrated that the similarity between isolates ranged from 0.5714 to 1. The different isolates were clustered in 3 karyotypes (A: 8 isolates; B: 6 isolates and C: 1 isolate). All isolates grouped in karyotype A presented a characteristic band of 730 kbp, whereas in the isolate of karyotype C the 810-kbp band was not observed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-08-01 | International Journal for Parasitology |