Osteogenic commitment and differentiation of human mesenchymal stem cells by low‐intensity pulsed ultrasound stimulation

6533b7d0fe1ef96bd125b8e3

RESEARCH PRODUCT

Osteogenic commitment and differentiation of human mesenchymal stem cells by low‐intensity pulsed ultrasound stimulation

Simona Fontana Milena Fini Angela De Luca Stefania Pagani Valeria Carina Francesca Monteleone Cesare Faldini Riccardo Alessandro Maria Sartori Stefania Setti Gianluca Giavaresi Viviana Costa

subject

Proteomics 0301 basic medicine Time Factors Ultrasonic Wave Transcription Factor Physiology Cellular differentiation Clinical Biochemistry Low-intensity pulsed ultrasound Osteogenesis Protein Interaction Maps Stem Cell Niche mesenchymal stem cell Cells Cultured Protein metabolic process proteomic analysi Mesenchymal Stromal Cell Reverse Transcriptase Polymerase Chain Reaction Osteogenesi Intracellular Signaling Peptides and Proteins Cell Differentiation Osteoblast proteomic analysis Flow Cytometry Cell biology RUNX2 Phenotype medicine.anatomical_structure Ultrasonic Waves osteoblast differentiation osteogenic commitment Protein Interaction Map Human Signal Transduction Homeobox protein NANOG low-intensity pulsed ultrasound Time Factor Cell Survival Enzyme-Linked Immunosorbent Assay Biology 03 medical and health sciences SOX2 medicine Humans Cell Lineage Mesenchymal stem cell Proteomic Mesenchymal Stem Cells Cell Biology 030104 developmental biology Gene Expression Regulation Intracellular Signaling Peptides and Protein Immunology Transcription Factors

description

Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm(2) LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7d, 14d, 21d). The hypothesis was that LIPUS would improve the osteogenic differentiation of hMSC and guarantying the maintenance of osteogenic committed fraction, as demonstrated by cell vitality and proteomic analysis. LIPUS stimulation (a) regulated the balance between osteoblast commitment and differentiation by specific networks (activations of RhoA/ROCK signaling and upregulation of Ribosome constituent/Protein metabolic process, Glycolysis/Gluconeogenesis, RNA metabolic process/Splicing and Tubulins); (b) allowed the maintenance of a few percentage of osteoblast precursors (21d CD73 + /CD90 + : 6%; OCT-3/4 + /NANOG + /SOX2 + : 10%); (c) induced the activation of osteogenic specific pathways shown by gene expression (early: ALPL, COL1A1, late: RUNX2, BGLAP, MAPK1/6) and related protein release (COL1a1, OPN, OC), in particular in the presence of osteogenic soluble factors able to mimic bone microenvironment. To summarize, LIPUS might be able to improve the osteogenic commitment of hMSCs in vitro, and, at the same time, enhance their osteogenic differentiation. This article is protected by copyright. All rights reserved.

year	journal	country	edition	language
2018-01-01	Journal of Cellular Physiology

https://doi.org/10.1002/jcp.26058