0000000000134467

AUTHOR

Stefania Setti

showing 4 related works from this author

Osteogenic commitment and differentiation of human mesenchymal stem cells by low‐intensity pulsed ultrasound stimulation

2018

Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm(2) LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7d, 14d, 21d). The hypothesis was that LIPUS would improve the osteogenic differentiation of hMSC and guarantying the maintenance of osteogenic committed fraction, as demonstrated by cell vitality and proteomic analysis. LIPUS stimulation (a) regulated the balance between osteoblast commitment and differentiation by specific network…

Proteomics0301 basic medicineTime FactorsUltrasonic WaveTranscription FactorPhysiologyCellular differentiationClinical BiochemistryLow-intensity pulsed ultrasoundOsteogenesisProtein Interaction MapsStem Cell Nichemesenchymal stem cellCells CulturedProtein metabolic processproteomic analysiMesenchymal Stromal CellReverse Transcriptase Polymerase Chain ReactionOsteogenesiIntracellular Signaling Peptides and ProteinsCell DifferentiationOsteoblastproteomic analysisFlow CytometryCell biologyRUNX2Phenotypemedicine.anatomical_structureUltrasonic Wavesosteoblast differentiationosteogenic commitmentProtein Interaction MapHumanSignal TransductionHomeobox protein NANOGlow-intensity pulsed ultrasoundTime FactorCell SurvivalEnzyme-Linked Immunosorbent AssayBiology03 medical and health sciencesSOX2medicineHumansCell LineageMesenchymal stem cellProteomicMesenchymal Stem CellsCell Biology030104 developmental biologyGene Expression RegulationIntracellular Signaling Peptides and ProteinImmunologyTranscription FactorsJournal of Cellular Physiology
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Effect of Low-Intensity Pulsed Ultrasound on Osteogenic Human Mesenchymal Stem Cells Commitment in a New Bone Scaffold

2017

Purpose Bone tissue engineering is helpful in finding alternatives to overcome surgery limitations. Bone growth and repair are under the control of biochemical and mechanical signals; therefore, in recent years several approaches to improve bone regeneration have been evaluated. Osteo-inductive biomaterials, stem cells, specific growth factors and biophysical stimuli are among those. The aim of the present study was to evaluate if low-intensity pulsed ultrasound stimulation (LIPUS) treatment would improve the colonization of an MgHA/Coll hybrid composite scaffold by human mesenchymal stem cells (hMSCs) and their osteogenic differentiation. LIPUS stimulation was applied to hMSCs cultured on …

0301 basic medicineMaterials scienceCellular differentiation0206 medical engineeringLow intensity pulsed ultrasoundBiomedical EngineeringBiophysicsBioengineeringHuman mesenchymal stem cell02 engineering and technologyLow-intensity pulsed ultrasoundHuman mesenchymal stem cellsBiomaterials03 medical and health sciencesTissue ScaffoldTissue engineeringTissue scaffoldsOsteogenesisOsteogenic differentiationHumansOriginal Research ArticleCells CulturedBone growthTissue EngineeringTissue ScaffoldsOsteogenesiMesenchymal stem cellCell DifferentiationMesenchymal Stem CellsBone scaffoldGeneral MedicineMgHA/Coll hybrid composite scaffold020601 biomedical engineeringMesenchymal Stem Cell030104 developmental biologyUltrasonic WavesLow intensity pulsed ultrasoundsHumanBiomedical engineeringJournal of Applied Biomaterials & Functional Materials
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Can Clinical and Surgical Parameters Be Combined to Predict How Long It Will Take a Tibia Fracture to Heal? A Prospective Multicentre Observational S…

2018

Background. Healing of tibia fractures occurs over a wide time range of months, with a number of risk factors contributing to prolonged healing. In this prospective, multicentre, observational study, we investigated the capability of FRACTING (tibia FRACTure prediction healING days) score, calculated soon after tibia fracture treatment, to predict healing time.Methods. The study included 363 patients. Information on patient health, fracture morphology, and surgical treatment adopted were combined to calculate the FRACTING score. Fractures were considered healed when the patient was able to fully weight-bear without pain.Results. 319 fractures (88%) healed within 12 months from treatment. Fo…

MaleGenetics and Molecular Biology (all)Immunology and Microbiology (all)lcsh:MedicineTibia fracture multicentre observational studyTibia Fracturefracture; healing; tibiaBiochemistry0302 clinical medicineOsteogenesisMedicineAdolescent; Adult; Female; Fracture Healing; Humans; Male; Middle Aged; Osteogenesis; Prospective Studies; Reproducibility of Results; Tibia; Tibial Fractures; Treatment Outcome; Young AdultProspective Studies030212 general & internal medicineYoung adultProspective cohort studyFracture Healing030222 orthopedicsGeneral MedicineMiddle Agedtibia fractureTreatment OutcomeAdolescent; Adult; Female; Fracture Healing; Humans; Male; Middle Aged; Osteogenesis; Prospective Studies; Reproducibility of Results; Tibia; Tibial Fractures; Treatment Outcome; Young Adult; Biochemistry Genetics and Molecular Biology (all); Immunology and Microbiology (all)Femaletibia fracturetibia FRACTure prediction healINGResearch ArticleAdultmedicine.medical_specialtyArticle SubjectAdolescentBone healingGeneral Biochemistry Genetics and Molecular BiologyNOYoung Adult03 medical and health sciencesHumansIn patientTibiaTime rangetibia FRACTure prediction healINGTibiaGeneral Immunology and Microbiologybusiness.industrylcsh:RReproducibility of ResultsSurgeryTibial FracturesObservational studybusinessBiochemistry Genetics and Molecular Biology (all); Immunology and Microbiology (all)
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miR-31-5p Is a LIPUS-Mechanosensitive MicroRNA that Targets HIF-1α Signaling and Cytoskeletal Proteins

2019

The roles of low-intensity pulsed ultrasound (LIPUS) and microRNAs (miRNAs) on hMSCs commitments have already been investigated

endocrine systemAngiogenesisregenerative medicineArticleCatalysisCell LineInorganic ChemistryRho family proteinlcsh:Chemistry03 medical and health sciences0302 clinical medicinemicroRNAmedicineHumansPhysical and Theoretical ChemistryBone regenerationCytoskeletonMolecular Biologylcsh:QH301-705.5Spectroscopy030304 developmental biologyMesenchymal stem cell0303 health sciencesmesenchymal stem cellsOsteoblastsChemistryhypoxiaOrganic ChemistryMesenchymal stem cellCell DifferentiationOsteoblastMicroRNAGeneral MedicineHypoxia-Inducible Factor 1 alpha Subunitequipment and suppliesUp-RegulationComputer Science ApplicationsCell biologymicroRNAsmir-31Cytoskeletal Proteinsmedicine.anatomical_structureUltrasonic Waveslcsh:Biology (General)lcsh:QD1-999030220 oncology & carcinogenesisMechanosensitive channelsInternational Journal of Molecular Sciences
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